HPLC Column, NP Silica, 3um, 2.1mm ID x 50mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Silica™ HPLC Columns are designed for the separation of hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured from ultra-high purity, low metal content 100Å silica that is not chemically modified, this stationary phase provides the native silica selectivity required for many traditional Normal Phase and HILIC applications. NP Silica™ is widely used as an alternative for USP L3 methods and for new analytical methods requiring the unique retention characteristics of unbonded silica.
82361-05P-2 is packed with 3 µm NP Silica™ particles in a 2.1 mm ID x 50 mm analytical format. The short 50 mm length is optimized for rapid analysis, method development, and reduced solvent consumption. The 3 µm particle size delivers higher chromatographic efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating backpressure should be expected. The narrow-bore format minimizes mobile phase usage while maintaining excellent separation performance.
NP Silica™ is commonly used for hydrophilic and polar compounds in both HILIC and Normal Phase chromatography. Because the stationary phase is completely unmodified, it provides the classic selectivity associated with bare silica and is often selected for established USP methods, method transfer projects, and applications requiring strong polar interactions. The 2.1 mm ID format is ideal when minimizing solvent consumption is important, but the low-flow requirements should be considered during method development.
Key Benefits
NP Silica™ stationary phase
USP L3 equivalent chemistry
Ultra-high purity silica
Low metal content silica
Unmodified silica surface
3 µm particle size
Higher efficiency than 5 µm particles
Sharper peak shapes
Suitable for HILIC separations
Suitable for Normal Phase chromatography
Typical Applications
USP L3 methods
HILIC chromatography
Normal Phase HPLC
Polar compound analysis
Hydrophilic compound analysis
Pharmaceutical analysis
Food and beverage testing
Environmental analysis
Method development
Established silica-based methods
Specifications
Product Type: Analytical HPLC Column
Stationary Phase: NP Silica™
USP Classification: L3
Chromatography Modes: HILIC and Normal Phase
Particle Size: 3 µm
Pore Size: 100Å
Column Dimensions: 2.1 mm ID x 50 mm
Recommended Flow Rate: 0.2–0.3 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for HILIC and Normal Phase chromatography
USP L3 equivalent chemistry
Unmodified silica stationary phase
Designed for hydrophilic and polar compounds
Low metal content, high-purity silica
Provides higher efficiency than comparable 5 µm columns
Generates higher operating backpressure than 5 µm particles
2.1 mm ID columns require an HPLC system capable of reliable low-flow operation
Low-volume detector flow cells are recommended for optimal performance
Frequently used for established silica-based methods
50 mm length minimizes solvent consumption and analysis time