Catalog Number - 82360-25P-3M

Cogent NP Diol™ Wide Pore HPLC Columns are designed for the separation of hydrophilic, polar, and larger molecular weight compounds using Normal Phase and HILIC chromatography modes. Manufactured using high-purity 300Å wide-pore silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase is not end capped and provides enhanced accessibility for larger molecules while maintaining the selectivity expected from a diol phase. NP Diol™ Wide Pore columns are widely used as an alternative for USP L20 methods and for analytical methods involving biomolecules, proteins, peptides, and other large molecular weight analytes.

82360-25P-3M is packed with 3 µm NP Diol™ Wide Pore particles in a 4.6 mm ID x 250 mm analytical format. The extended 250 mm column length provides maximum retention, chromatographic resolution, and peak capacity for demanding analytical separations. The 3 µm particle size delivers higher efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating pressure should be expected. This format is ideal when the highest degree of separation is required for large molecules, complex samples, and closely related compounds.

The NP Diol™ Wide Pore stationary phase is commonly selected for hydrophilic and polar compounds analyzed by HILIC and Normal Phase chromatography, particularly when larger molecules require enhanced pore accessibility. It is frequently used as a replacement for unmodified silica columns because it offers improved robustness and broader solvent compatibility while maintaining strong polar selectivity. The 300Å pore structure is especially beneficial for proteins, peptides, biomolecules, and other large analytes that may not be effectively separated on standard pore-size materials.

Key Benefits

• NP Diol™ Wide Pore stationary phase

• USP L20 equivalent chemistry

• High-purity 300Å silica

• Diol bonded phase

• Wide pore design for large molecules

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Sharper peak shapes

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

Typical Applications

• USP L20 methods

• HILIC chromatography

• Normal Phase HPLC

• Large molecule analysis

• Protein analysis

• Peptide analysis

• Biomolecule separations

• Polar compound analysis

• Method development

• Complex analytical separations

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Diol™ Wide Pore

• USP Classification: L20

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 300Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L20 equivalent chemistry

• Not end capped

• Designed for hydrophilic and polar compounds

• Wide pore 300Å silica provides improved accessibility for larger molecules

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• Compatible with standard analytical HPLC systems capable of supporting 3 µm particle columns

• Often selected as a more robust alternative to unmodified silica columns

• Broad solvent compatibility

• 250 mm length provides maximum retention, resolution, and peak capacity

• Ideal for large molecule separations and challenging analytical methods

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