Catalog Number - 82360-25P

Cogent NP Diol™ HPLC Columns are designed for the separation of hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured using high-purity 100Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase is not end capped and provides versatile selectivity for compounds that may be difficult to retain using conventional reversed-phase chromatography. NP Diol™ is widely used as an alternative for USP L20 methods and for developing robust analytical methods requiring enhanced retention of polar analytes.

82360-25P is packed with 3 µm NP Diol™ particles in a 4.6 mm ID x 250 mm analytical format. The 250 mm column length provides maximum retention, chromatographic resolution, and peak capacity for demanding analytical separations. The 3 µm particle size delivers higher efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating pressure should be expected. This format is ideal when the highest degree of separation is required for complex mixtures and closely related compounds.

The NP Diol™ stationary phase is commonly selected for hydrophilic and polar compounds analyzed by HILIC and Normal Phase chromatography. It is frequently used as a replacement for unmodified silica columns because it offers improved robustness and broader solvent compatibility while maintaining useful selectivity for polar analytes. The extended 250 mm length provides maximum resolving power for challenging analyses while preserving excellent reproducibility and method robustness.

Key Benefits

• NP Diol™ stationary phase

• USP L20 equivalent chemistry

• High-purity 100Å silica

• Diol bonded phase

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Sharper peak shapes

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

• Maximum resolution and peak capacity

Typical Applications

• USP L20 methods

• HILIC chromatography

• Normal Phase HPLC

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• Food and beverage testing

• Environmental testing

• Method development

• Complex analytical separations

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Diol™

• USP Classification: L20

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L20 equivalent chemistry

• Not end capped

• Designed for hydrophilic and polar compounds

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• Compatible with standard analytical HPLC systems capable of supporting 3 µm particle columns

• Often selected as a more robust alternative to unmodified silica columns

• Broad solvent compatibility

• 250 mm length provides maximum retention, resolution, and peak capacity

• Ideal for complex separations and challenging analytical methods

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