Catalog Number - 82360-15P

Cogent NP Diol™ HPLC Columns are designed for the separation of hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured using high-purity 100Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase is not end capped and provides versatile selectivity for compounds that may be difficult to retain using conventional reversed-phase chromatography. NP Diol™ is widely used as an alternative for USP L20 methods and for developing robust analytical methods requiring enhanced retention of polar analytes.

82360-15P is packed with 3 µm NP Diol™ particles in a 4.6 mm ID x 150 mm analytical format. The 150 mm column length provides enhanced resolution and peak capacity compared to shorter formats, making it ideal for more demanding analytical separations. The 3 µm particle size delivers higher efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating pressure should be expected. This configuration is one of the most popular NP Diol™ formats due to its excellent balance of retention, selectivity, and chromatographic performance.

The NP Diol™ stationary phase is commonly selected for hydrophilic and polar compounds analyzed by HILIC and Normal Phase chromatography. It is frequently used as a replacement for unmodified silica columns because it offers improved robustness and broader solvent compatibility while maintaining useful selectivity for polar analytes. The additional column length provides increased resolving power for complex samples and challenging separations while maintaining reliable and reproducible performance.

Key Benefits

• NP Diol™ stationary phase

• USP L20 equivalent chemistry

• High-purity 100Å silica

• Diol bonded phase

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Sharper peak shapes

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

• Enhanced resolution and peak capacity

Typical Applications

• USP L20 methods

• HILIC chromatography

• Normal Phase HPLC

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• Food and beverage testing

• Environmental testing

• Method development

• Replacement of silica columns

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Diol™

• USP Classification: L20

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 150 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L20 equivalent chemistry

• Not end capped

• Designed for hydrophilic and polar compounds

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• Compatible with standard analytical HPLC systems capable of supporting 3 µm particle columns

• Often selected as a more robust alternative to unmodified silica columns

• Broad solvent compatibility

• 150 mm length provides enhanced resolution and peak capacity

• One of the most popular NP Diol™ column formats

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