Catalog Number - 82360-10P-2

Cogent NP Diol™ HPLC Columns are designed for the separation of hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured using high-purity 100Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase is not end capped and provides versatile selectivity for compounds that may be difficult to retain using conventional reversed-phase chromatography. NP Diol™ is widely used as an alternative for USP L20 methods and for developing robust analytical methods requiring enhanced retention of polar analytes.

82360-10P-2 is packed with 3 µm NP Diol™ particles in a 2.1 mm ID x 100 mm analytical format. The narrow-bore design reduces solvent consumption while maintaining excellent chromatographic efficiency. The 3 µm particle size delivers higher efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating pressure should be expected. The 100 mm column length provides an excellent balance between resolution and analysis speed, making it suitable for routine analytical workflows.

The NP Diol™ stationary phase is commonly selected for hydrophilic and polar compounds analyzed by HILIC and Normal Phase chromatography. It is frequently used as a replacement for unmodified silica columns because it offers improved robustness and broader solvent compatibility while maintaining useful selectivity for polar analytes. The 2.1 mm ID format is ideal when reduced solvent consumption and efficient separations are desired, but this chromatography mode is generally not preferred for LC-MS applications.

Key Benefits

• NP Diol™ stationary phase

• USP L20 equivalent chemistry

• High-purity 100Å silica

• Diol bonded phase

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Sharper peak shapes

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

• Improved robustness versus unmodified silica

Typical Applications

• USP L20 methods

• HILIC chromatography

• Normal Phase HPLC

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• Food and beverage testing

• Environmental testing

• Method development

• Replacement of silica columns

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Diol™

• USP Classification: L20

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 100Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L20 equivalent chemistry

• Not end capped

• Designed for hydrophilic and polar compounds

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• 2.1 mm ID columns require an HPLC system capable of reliable low-flow operation

• Low-volume detector flow cells are recommended for optimal performance

• Often selected as a more robust alternative to unmodified silica columns

• Broad solvent compatibility

• 100 mm length provides an excellent balance of speed and resolution

• Generally not recommended for LC-MS applications

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