Catalog Number - 82360-05P-2-3M

Cogent NP Diol™ Wide Pore HPLC Columns are designed for the separation of hydrophilic, polar, and larger molecular weight compounds using Normal Phase and HILIC chromatography modes. Manufactured using high-purity 300Å wide-pore silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase is not end capped and provides excellent accessibility for larger molecules while maintaining the selectivity expected from a diol phase. NP Diol™ Wide Pore columns are widely used as an alternative for USP L20 methods and for analytical methods involving biomolecules, polymers, and other larger analytes.

82360-05P-2-3M is packed with 3 µm NP Diol™ Wide Pore particles in a 2.1 mm ID x 50 mm analytical format. The short 50 mm length is optimized for rapid analysis, method development, and reduced solvent consumption. The 3 µm particle size provides higher efficiency and sharper peaks than comparable 5 µm columns, although higher operating pressures should be expected. The narrow-bore format further reduces mobile phase consumption while maintaining excellent chromatographic efficiency.

The NP Diol™ Wide Pore stationary phase is commonly selected for hydrophilic and polar compounds analyzed by HILIC and Normal Phase chromatography, particularly when larger molecules require enhanced pore accessibility. It is frequently used as a replacement for unmodified silica columns because it offers improved robustness and broader solvent compatibility while maintaining strong polar selectivity. The 300Å pore structure makes this column especially suitable for larger molecular weight compounds that may not be optimally separated on standard pore-size materials.

Key Benefits

• NP Diol™ Wide Pore stationary phase

• USP L20 equivalent chemistry

• High-purity 300Å silica

• Diol bonded phase

• Wide pore design for larger molecules

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Sharper peak shapes

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

Typical Applications

• USP L20 methods

• HILIC chromatography

• Normal Phase HPLC

• Large molecule analysis

• Biomolecule separations

• Protein and peptide analysis

• Hydrophilic compound analysis

• Polar compound analysis

• Method development

• Replacement of silica columns

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Diol™ Wide Pore

• USP Classification: L20

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 300Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L20 equivalent chemistry

• Not end capped

• Designed for hydrophilic and polar compounds

• Wide pore 300Å silica provides improved accessibility for larger molecules

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• 2.1 mm ID columns require an HPLC system capable of reliable low-flow operation

• Low-volume detector flow cells are recommended for optimal performance

• Often selected as a more robust alternative to unmodified silica columns

• 50 mm length minimizes solvent consumption and analysis time

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