Catalog Number - 82360-05P-2

Cogent NP Diol™ HPLC Columns are designed for the separation of polar and hydrophilic compounds using Normal Phase and HILIC chromatography modes. Manufactured using high-purity 100Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase is not end capped and provides versatile selectivity for compounds that may be difficult to retain using conventional reversed-phase chromatography. NP Diol™ is widely used as an alternative for USP L20 methods and for developing robust analytical methods requiring enhanced polar compound retention.

82360-05P-2 is packed with 3 µm NP Diol™ particles in a 2.1 mm ID x 50 mm analytical format. The short 50 mm column length is optimized for rapid analysis, method development, and reduced solvent consumption. The 3 µm particle size provides higher efficiency and sharper peaks than comparable 5 µm columns, although higher operating pressures should be expected. The narrow-bore format is especially useful when minimizing mobile phase consumption is a priority.

The NP Diol™ stationary phase is commonly selected for hydrophilic and polar compounds in HILIC and Normal Phase chromatography. It is often used as a replacement for unmodified silica columns because it offers improved robustness and compatibility with a broader range of solvents while maintaining useful selectivity for polar analytes. Unlike many 2.1 mm ID columns, this format is generally not recommended for LC-MS applications due to the chromatography mode and typical mobile phase considerations associated with these methods.

Key Benefits

• NP Diol™ stationary phase

• USP L20 equivalent chemistry

• High-purity 100Å silica

• Diol bonded phase

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Sharper peak shapes

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

• Improved robustness versus unmodified silica

Typical Applications

• USP L20 methods

• HILIC chromatography

• Normal Phase HPLC

• Polar compound analysis

• Hydrophilic compound analysis

• Carbohydrate analysis

• Pharmaceutical analysis

• Food and beverage testing

• Method development

• Replacement of silica columns

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Diol™

• USP Classification: L20

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 100Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L20 equivalent chemistry

• Not end capped

• Designed for hydrophilic and polar compounds

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• 2.1 mm ID columns require an HPLC system capable of reliable low-flow operation

• Low-volume detector flow cells are recommended for optimal performance

• Often selected as a more robust alternative to unmodified silica columns

• Broad solvent compatibility

• Not generally recommended for LC-MS applications

• 50 mm length minimizes solvent consumption and analysis time

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