Catalog Number - 82360-05P

Cogent NP Diol™ HPLC Columns are designed for the separation of polar and hydrophilic compounds using Normal Phase and HILIC chromatography modes. Manufactured using high-purity 100Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase is not end capped and provides versatile selectivity for compounds that may be difficult to retain using conventional reversed-phase chromatography. NP Diol™ is widely used as an alternative for USP L20 methods and for developing robust analytical methods requiring enhanced polar compound retention.

82360-05P is packed with 3 µm NP Diol™ particles in a 4.6 mm ID x 50 mm analytical format. The short 50 mm column length is optimized for rapid analysis, method development, and high-throughput screening applications. The 3 µm particle size provides higher efficiency and sharper peaks than comparable 5 µm columns, although system backpressure will be higher and should be considered when selecting operating conditions.

The NP Diol™ stationary phase is commonly selected for hydrophilic and polar compounds in HILIC and Normal Phase chromatography. It is often used as a replacement for unmodified silica columns because it offers improved robustness and compatibility with a broader range of solvents while maintaining useful selectivity for polar analytes. This column is well suited for pharmaceutical, biochemical, food, and general analytical applications where retention of polar compounds is required.

Key Benefits

• NP Diol™ stationary phase

• USP L20 equivalent chemistry

• High-purity 100Å silica

• Diol bonded phase

• 3 µm particle size

• Higher efficiency than 5 µm particles

• Sharper peak shapes

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

• Improved robustness versus unmodified silica

Typical Applications

• USP L20 methods

• HILIC chromatography

• Normal Phase HPLC

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• Food and beverage testing

• Biochemical analysis

• Method development

• Replacement of silica columns

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Diol™

• USP Classification: L20

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 3 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L20 equivalent chemistry

• Not end capped

• Designed for hydrophilic and polar compounds

• Provides higher efficiency than comparable 5 µm columns

• Generates higher operating backpressure than 5 µm particles

• Compatible with standard analytical HPLC systems capable of the required pressure range

• Often selected as a more robust alternative to unmodified silica columns

• Broad solvent compatibility

• 50 mm length minimizes solvent consumption and analysis time

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