Catalog Number - 82161-10P

Cogent NP Silica™ UHPLC Columns are designed for high-efficiency analytical separations using modern UHPLC and UPLC-type systems. Manufactured from ultra-high purity, low metal content, 100Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for HILIC and Normal Phase chromatography. Equivalent to USP L3 silica chemistry, it is an excellent choice for developing new UHPLC methods and for transferring or optimizing established USP methods that specify L3 stationary phases.

82161-10P is packed with 1.8 µm NP Silica™ particles in a 4.6 mm ID x 100 mm analytical UHPLC column format. The small particle size produces exceptionally high efficiency, increased peak capacity, and enhanced chromatographic resolution compared to larger particle columns. The 100 mm length provides additional resolving power while maintaining the speed advantages associated with UHPLC technology, making it an excellent choice for demanding analytical separations and method development applications.

This column is especially well suited for HILIC separations of polar and hydrophilic compounds. The unmodified silica surface provides strong retention of polar analytes, making it an excellent choice for the analysis of pharmaceuticals, metabolites, amino acids, organic acids, carbohydrates, and other compounds that are often difficult to retain using traditional reversed-phase methods. The combination of ultra-high purity silica and 1.8 µm particle technology delivers outstanding resolution, reproducibility, and throughput for advanced analytical workflows while reducing solvent consumption and operating costs.

Key Benefits

• NP Silica™ stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 100Å pore size

• 1.8 µm particle size

• High efficiency separations

• Increased peak capacity

• Excellent for HILIC separations of polar compounds

Typical Applications

• UHPLC analysis

• UPLC-type systems

• HILIC chromatography

• Normal Phase chromatography

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• Quality control testing

• Method development

• High-throughput analysis

Specifications

• Product Type: UHPLC Column

• Stationary Phase: NP Silica™

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 1.8 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Recommended Flow Rate: 0.8–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed exclusively for UHPLC and UPLC-type systems

• UPLC is a trademarked brand designation of Waters Corporation

• Excellent for HILIC separations of polar and hydrophilic compounds

• Suitable for Normal Phase chromatography

• USP L3 equivalent stationary phase

• Unmodified silica surface chemistry

• Manufactured from ultra-high purity, low metal content silica

• Provides significantly higher efficiency than larger particle columns

• Ideal for reducing run times while maintaining or improving resolution

• Excellent for method development and method optimization

• Reduced solvent consumption and increased sample throughput

• Requires a UHPLC system capable of operating at the elevated pressures generated by 1.8 µm particles

• Samples should be filtered using a 0.2 µm syringe filter prior to injection

• 100 mm length provides increased resolving power for complex separations

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