Catalog Number - 82061-10P-3M

Cogent NP Silica™ Wide Pore HPLC Columns are designed for the separation of large hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured from ultra-high purity, low metal content 300Å silica that is not chemically modified, this stationary phase provides the native silica selectivity required for challenging separations involving larger molecules. NP Silica™ Wide Pore columns are ideal for developing analytical, preparative, and purification methods involving proteins, peptides, biomolecules, and other high molecular weight compounds.

82061-10P-3M is packed with 10 µm NP Silica™ Wide Pore particles in a 4.6 mm ID x 100 mm analytical format. The larger 10 µm particle size generates significantly lower backpressure than smaller particle columns, allowing operation at higher flow rates while maintaining reliable chromatographic performance. The 100 mm length provides an excellent balance of retention, resolution, and analysis time, making it well suited for both scouting studies and routine method development involving large molecules.

NP Silica™ Wide Pore 10 µm columns are very often used as scouting columns for preparative chromatography, purification methods, and process scale-up projects. The 300Å pore structure provides improved accessibility for proteins, peptides, and other high molecular weight analytes, while the 100 mm format offers greater resolving power than shorter scouting columns. Because the stationary phase chemistry remains consistent, methods developed on this column can often be scaled directly to larger preparative and purification columns with predictable chromatographic behavior, helping reduce development time and streamline the scale-up process.

Key Benefits

• NP Silica™ Wide Pore stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 300Å wide pore design

• 10 µm particle size

• Lower operating backpressure

• Ideal for preparative method scouting

• Suitable for HILIC and Normal Phase chromatography

Typical Applications

• Large molecule analysis

• Protein analysis

• Peptide analysis

• Biomolecule separations

• HILIC chromatography

• Normal Phase HPLC

• Preparative method development

• Purification method scouting

• Process scale-up studies

• Method transfer to preparative columns

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Silica™ Wide Pore

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 300Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Recommended Flow Rate: 1.0–3.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent chemistry

• Unmodified silica stationary phase

• Low metal content, high-purity silica

• Designed for large hydrophilic and polar compounds

• Wide pore 300Å silica provides improved accessibility for proteins, peptides, and other large molecules

• Produces lower operating backpressure than smaller particle columns

• Supports higher flow rates than 3 µm and 5 µm particle columns

• Frequently used as a scouting column for preparative methods and purification processes

• Excellent for large molecule method development and process scale-up

• Methods can often be transferred directly to larger preparative and purification columns with the same stationary phase

• Compatible with standard analytical HPLC systems

• 100 mm length provides an excellent balance of speed and resolution

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