HPLC Column, NP Silica, Wide Pore, 10um, 2.1mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Silica™ Wide Pore HPLC Columns are designed for the separation of large hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured from ultra-high purity, low metal content 300Å silica that is not chemically modified, this stationary phase provides the native silica selectivity required for large molecule separations while maintaining the retention characteristics expected from unbonded silica. NP Silica™ Wide Pore columns are ideal for developing analytical, preparative, and purification methods involving proteins, peptides, biomolecules, and other high molecular weight compounds.
82061-05P-2-3M is packed with 10 µm NP Silica™ Wide Pore particles in a 2.1 mm ID x 50 mm analytical format. The larger 10 µm particle size generates substantially lower backpressure than smaller particle columns, allowing operation at higher linear velocities while maintaining efficient separations. The short 50 mm length is particularly useful for rapid method development, screening studies, and solvent-efficient scouting applications.
NP Silica™ Wide Pore 10 µm columns are very often used as scouting columns for preparative chromatography, purification methods, and process scale-up projects involving large molecules. The 300Å pore structure provides improved accessibility for proteins, peptides, and other large analytes, while the 50 mm format enables rapid evaluation of selectivity and mobile phase conditions. Because the stationary phase chemistry remains consistent, methods developed on this column can often be scaled directly to larger preparative and purification columns with predictable chromatographic behavior, reducing development time and simplifying scale-up.
Key Benefits
NP Silica™ Wide Pore stationary phase
USP L3 equivalent chemistry
Ultra-high purity silica
Low metal content silica
Unmodified silica surface
300Å wide pore design
10 µm particle size
Lower operating backpressure
Ideal for preparative method scouting
Suitable for HILIC and Normal Phase chromatography
Typical Applications
Large molecule analysis
Protein analysis
Peptide analysis
Biomolecule separations
HILIC chromatography
Normal Phase HPLC
Preparative method development
Purification method scouting
Process scale-up studies
Method transfer to preparative columns
Specifications
Product Type: Analytical HPLC Column
Stationary Phase: NP Silica™ Wide Pore
USP Classification: L3
Chromatography Modes: HILIC and Normal Phase
Particle Size: 10 µm
Pore Size: 300Å
Column Dimensions: 2.1 mm ID x 50 mm
Recommended Flow Rate: 0.2–0.3 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for HILIC and Normal Phase chromatography
USP L3 equivalent chemistry
Unmodified silica stationary phase
Low metal content, high-purity silica
Designed for large hydrophilic and polar compounds
Wide pore 300Å silica provides improved accessibility for proteins, peptides, and other large molecules
Produces lower operating backpressure than smaller particle columns
Frequently used as a scouting column for preparative methods and purification processes
Excellent for large molecule method development and process scale-up
Methods can often be transferred directly to larger preparative and purification columns with the same stationary phase
2.1 mm ID columns require an HPLC system capable of reliable low-flow operation
50 mm length minimizes solvent consumption and analysis time