HPLC Column, NP Diol, Wide Pore, 10um, 4.6mm ID x 250mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Diol™ Wide Pore HPLC Columns are designed for Normal Phase and HILIC separations of larger hydrophilic and polar compounds. Manufactured using ultra-high purity 300Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reliable retention, excellent reproducibility, and improved accessibility for larger analytes. NP Diol™ Wide Pore is an excellent alternative for USP L20 methods and is well suited for the analysis of larger molecules that require enhanced pore access.
82060-25P-3M is packed with 10 µm NP Diol™ Wide Pore particles in a 4.6 mm ID x 250 mm format with a 300Å pore size. The larger 10 µm particle size allows operation at higher flow rates while producing lower backpressure than smaller particle columns. The extended 250 mm column length provides maximum resolving power and increased peak capacity, making it ideal for complex separations, method optimization, and analytical applications involving larger molecules.
The NP Diol™ Wide Pore stationary phase is non-end-capped and is frequently selected as an alternative to unmodified silica columns due to its enhanced durability and broader solvent compatibility. The 300Å pore structure improves mass transfer and accessibility for larger molecules while maintaining effective retention of hydrophilic and polar compounds in both HILIC and Normal Phase chromatography. This column is particularly useful for large molecule applications requiring maximum resolution and reproducible separations.
Key Benefits
NP Diol™ Wide Pore stationary phase
USP L20 equivalent chemistry
Ultra-high purity 300Å silica support
Terminal diol functional group
Non-end-capped chemistry
300Å wide pore structure
10 µm particle size
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced accessibility for larger molecules
Typical Applications
Normal Phase HPLC
HILIC chromatography
USP L20 methods
Large molecule analysis
Polar compound separations
Hydrophilic compound analysis
Carbohydrate analysis
Biopolymer analysis
Method development
Quality control testing
Specifications
Product Type: HPLC Column
Stationary Phase: NP Diol™ Wide Pore
Chromatography Mode: Normal Phase / HILIC
USP Classification: L20
Particle Size: 10 µm
Pore Size: 300Å
Column Dimensions: 4.6 mm ID x 250 mm
Recommended Flow Rate: 1.0–2.0 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for Normal Phase chromatography
Suitable for HILIC applications
USP L20 equivalent chemistry
Non-end-capped stationary phase
Designed for larger hydrophilic and polar compounds
Wide pore structure improves accessibility for larger analytes
Alternative to unmodified silica columns
Compatible with a wide range of solvents
10 µm particles allow higher flow rates with lower backpressure
250 mm length provides maximum retention, resolution, and peak capacity