Catalog Number - 75025-15P-2

Cogent HPS Cyano™ HPLC Columns are designed for versatile reversed-phase separations of polar and neutral compounds requiring moderate retention, rapid equilibration, and reproducible chromatographic performance. Manufactured using a monofunctional three-carbon ligand with a terminal cyano functional group bonded to high-purity 120Å silica and fully end capped, this stationary phase offers unique selectivity characteristics that are widely used as an alternative for USP L10 methods and for new method development. The HPS product line was developed to provide a high-value option for laboratories seeking performance characteristics comparable to established cyano phases from leading manufacturers.

75025-15P-2 is packed with 5 µm HPS Cyano™ particles in a 2.1 mm ID x 150 mm analytical format. The 5 µm particle size provides reliable chromatographic efficiency with lower operating backpressure than smaller particles, while the 150 mm column length delivers enhanced resolution and peak capacity for more challenging separations. The narrow-bore format reduces solvent consumption and is particularly beneficial for LC-MS applications where improved sensitivity and efficient mobile phase utilization are important.

The HPS Cyano™ stationary phase is commonly used for polar and neutral compounds analyzed using reversed-phase chromatography. Compared to non-modified silica columns, the cyano phase provides faster equilibration between runs and shorter retention times while maintaining excellent selectivity. This column is frequently selected for LC-MS methods, pharmaceutical analysis, method development, and analytical workflows requiring enhanced resolution together with moderate retention characteristics.

Key Benefits

• HPS Cyano™ stationary phase

• USP L10 equivalent chemistry

• Fully end capped

• Monofunctional cyano bonded phase

• High-purity 120Å silica

• 5 µm particle size

• Lower operating backpressure

• Faster equilibration between runs

• Shorter retention than non-modified silica

• Enhanced resolution with LC-MS compatibility

Typical Applications

• USP L10 methods

• LC-MS and LC-MS/MS methods

• Reversed-phase HPLC

• Polar compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Method validation

• Routine quality control

• Preparative method scouting

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: HPS Cyano™

• USP Classification: L10

• Chromatography Mode: Reversed Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for reversed-phase chromatography

• USP L10 equivalent chemistry

• Fully end capped stationary phase

• Designed for polar and neutral compounds

• Provides faster equilibration than non-modified silica columns

• Delivers shorter retention times than silica phases

• Well suited for LC-MS applications

• 2.1 mm ID columns require an HPLC system capable of reliable low-flow operation

• Low-volume detector flow cells are recommended for optimal performance

• Frequently compared with established cyano columns from Inertsil™ and Luna™ product families

• 150 mm length provides enhanced resolution and peak capacity for challenging separations