HPLC Column, C30, 5um, 4.6mm ID x 250mm Length, 200A. Cogent Brand. 1 EA.
Additional Info:
The 72530‑25P Cogent C30™ HPLC Column is engineered for maximum‑resolution, shape‑selective separation of long‑chain hydrophobic compounds, delivering excellent performance for positional and geometric isomers that are difficult to resolve using conventional C18 phases.
This column is built with a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing enhanced hydrophobic interaction and a unique selectivity profile that enables separation of closely related compounds under reversed‑phase conditions.
The 200 Å pore size allows efficient interaction with larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining consistent chromatographic performance.
✅ Comparable selectivity and application performance to YMC® C30 columns, making it an excellent alternative for USP L62 methods and equivalent analytical workflows
✅ One of the most popular C30 columns for high‑resolution separations
250 mm Length – Maximum Resolution & Separation Power
The 250 mm length provides the highest resolving power in the C30 analytical range, allowing separation of complex mixtures and trace components with greater clarity.
Maximum peak capacity for complex samples
Superior separation of positional and geometric isomers
Improved detection of trace impurities
✅ Use when 150 mm columns cannot achieve required resolution or purity
Flow Rate & System Requirements (4.6 mm ID, 5 µm)
⚠️ Compatible with standard HPLC systems, but longer column length increases system pressure
Typical flow rate range: ~0.8–1.5 mL/min
Recommended starting range: ~1.0–1.2 mL/min
Increased backpressure compared to shorter columns
Extra caution required with viscous mobile phases
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose C30 for Hydrophobic Separations
Maximum Shape Selectivity
Resolves positional and geometric isomers
Provides separation capabilities beyond C18
Optimized for Larger Molecules
200 Å pore supports complex analytes
Ideal for carotenoids, vitamins, and peptides
Reliable Alternative to Normal Phase
Avoids instability and reproducibility issues
Maintains consistent reversed‑phase performance
Cogent C30™ Advantages
Extended C30 Ligand
Stronger hydrophobic interaction vs C18
Unique selectivity for long‑chain compounds
High Purity Silica
Consistent column‑to‑column performance
Reliable chromatographic reproducibility
Method Transfer Capability
Comparable to YMC® C30 column chemistry and applications
Suitable for transferring L62 methods with minimal adjustment
Typical Applications
Carotenoid and vitamin analysis
Isomer and positional compound separations
Peptide and large molecule separations
Hydrophobic compound profiling
High‑resolution method development
Important Usage Notes
Designed for standard analytical HPLC systems (4.6 mm ID)
Not end‑capped (intentional for enhanced selectivity)
Higher backpressure due to column length
Ideal for maximum‑resolution analytical workflows