HPLC Column, NP Amino, Wide Pore, 5um, 2.1mm ID x 150mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Amino™ Wide Pore HPLC Columns are designed for Normal Phase and HILIC separations of larger polar compounds that require increased pore accessibility. Manufactured using ultra-high purity silica bonded with a three-carbon propyl linker and terminal amino functionality, this stationary phase provides the selectivity needed for retention and separation of complex sugars, larger organic acids, and other highly polar molecules. NP Amino™ Wide Pore is an excellent alternative for USP L8 methods involving larger analytes and for new method development requiring enhanced access to the stationary phase.
68540-15P-2-3M is packed with 5 µm NP Amino™ Wide Pore particles in a 2.1 mm ID x 150 mm format with a 300Å pore size. The large pore structure improves accessibility and mass transfer for larger molecules while maintaining the characteristic retention mechanisms of amino-based stationary phases. The narrow-bore 2.1 mm ID format reduces solvent consumption and typically operates at flow rates of approximately 0.2–0.3 mL/min. The HPLC system must be capable of delivering stable and accurate low-flow performance for optimum results.
The amino stationary phase is not end capped and performs well for complex sugars, larger compounds containing carboxylic acid groups, and other highly polar analytes using Normal Phase or HILIC chromatographic modes. The 300Å pore size enhances performance with larger molecules that may be partially excluded from smaller pore stationary phases. This popular narrow-bore format provides excellent separations for larger molecules while requiring a pump capable of precise low-flow delivery and a detector flow cell optimized for reduced flow rates and low internal volume.
Key Benefits
NP Amino™ Wide Pore stationary phase
USP L8 equivalent chemistry
Ultra-high purity silica support
Three-carbon propyl linker with terminal amino group
Not end capped
300Å pore size
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced accessibility for larger molecules
Excellent resolution for complex polar separations
Typical Applications
Normal Phase HPLC
HILIC chromatography
USP L8 methods
Complex sugar analysis
Carbohydrate separations
Organic acid analysis
Polar compound separations
Large molecule analysis
Method development
Analytical method scouting
Specifications
Product Type: HPLC Column
Stationary Phase: NP Amino™ Wide Pore
Chromatography Mode: Normal Phase / HILIC
USP Classification: L8
Particle Size: 5 µm
Pore Size: 300Å
Column Dimensions: 2.1 mm ID x 150 mm
Recommended Flow Rate: 0.2–0.3 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for Normal Phase chromatography
Suitable for HILIC applications
USP L8 equivalent chemistry
Not end capped
Designed for larger polar molecules and complex sugars
Wide pore structure improves accessibility for larger analytes
Packed and shipped in normal-phase solvents
Requires an HPLC system capable of reliable low-flow operation
Requires a detector flow cell optimized for low-volume, low-flow applications
150 mm length provides enhanced retention and resolution
Provides an alternative retention mechanism to reversed-phase chromatography