HPLC Column, RP Phenyl Hexyl, 5um, 4.6mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl Hexyl™ HPLC Columns are designed for reversed-phase separations where enhanced aromatic selectivity and strong π-π interactions are desired. Manufactured using ultra-high purity silica bonded with a six-carbon linker and terminal Phenyl group, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. RP Phenyl Hexyl™ is an excellent alternative for certain USP L11 methods that specify or benefit from Phenyl Hexyl selectivity.
68539-25P is packed with 5 µm RP Phenyl Hexyl™ particles in a 4.6 mm ID x 250 mm format with a 100Å pore size. The 5 µm particle size provides lower operating backpressure than smaller particles while maintaining excellent efficiency and reproducibility. The extended 250 mm column length provides maximum resolving power and increased peak capacity, making it ideal for complex separations, method development, and analytical methods requiring enhanced resolution.
The six-carbon linker positions the Phenyl group farther from the silica surface, increasing π-π interactions while reducing the influence of residual silanol activity. This enhanced selectivity can improve retention and separation of many aromatic and moderately polar analytes including positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. RP Phenyl Hexyl™ columns are often evaluated as an alternative to other Phenyl Hexyl stationary phases used in USP L11 methods, including Restek™ Phenyl Hexyl columns. For optimized π-π interactions, methanol is often preferred over acetonitrile as the organic modifier. This column size is frequently used in USP monograph methods.
Key Benefits
RP Phenyl Hexyl™ stationary phase
USP L11 equivalent chemistry
Ultra-high purity silica support
Fully double end-capped
Six-carbon linker with terminal Phenyl group
100Å pore size
Suitable for reversed-phase chromatography
Enhanced π-π interactions
Alternative selectivity to conventional Phenyl phases
Maximum resolution for complex separations
Typical Applications
Reversed-phase HPLC
USP L11 methods
Aromatic compound separations
Positional isomer separations
Tocopherol analysis
Flavonoid analysis
Polynuclear aromatic hydrocarbon analysis
Nitro-aromatic compound separations
Active pharmaceutical ingredient (API) analysis
Natural product analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl Hexyl™
Chromatography Mode: Reversed Phase
USP Classification: L11
Particle Size: 5 µm
Pore Size: 100Å
Column Dimensions: 4.6 mm ID x 250 mm
Recommended Flow Rate: 1.0–2.0 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for reversed-phase chromatography
USP L11 equivalent chemistry
Fully double end-capped
Enhanced π-π interactions improve aromatic selectivity
Six-carbon linker increases distance from the silica surface
Excellent for aromatic, polar, and structurally related compounds
Often evaluated as an alternative to Restek™ Phenyl Hexyl columns in USP L11 methods
250 mm length provides maximum resolution and peak capacity
Methanol is often preferred over acetonitrile when maximizing π-π interactions
Frequently used in USP monograph methods