Catalog Number - 68539-15P-2

Cogent RP Phenyl Hexyl™ HPLC Columns are designed for reversed-phase separations where enhanced aromatic selectivity and strong π-π interactions are desired. Manufactured using ultra-high purity silica bonded with a six-carbon linker and terminal Phenyl group, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. RP Phenyl Hexyl™ is an excellent alternative for certain USP L11 methods that specify or benefit from Phenyl Hexyl selectivity.

68539-15P-2 is packed with 5 µm RP Phenyl Hexyl™ particles in a 2.1 mm ID x 150 mm format with a 100Å pore size. The 5 µm particle size provides low operating backpressure and excellent reproducibility for LC-MS compatible methods. The 2.1 mm ID format reduces solvent consumption while improving mass spectrometry sensitivity. Typical operating flow rates are approximately 0.2–0.3 mL/min, and the HPLC system must be capable of delivering stable and accurate low-flow performance for optimum results.

The six-carbon linker positions the Phenyl group farther from the silica surface, increasing π-π interactions while reducing the influence of residual silanol activity. This enhanced selectivity can improve retention and separation of many aromatic and moderately polar analytes including positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. RP Phenyl Hexyl™ columns are often evaluated as an alternative to other Phenyl Hexyl stationary phases used in USP L11 methods, including Restek™ Phenyl Hexyl columns. For optimized π-π interactions, methanol is often preferred over acetonitrile as the organic modifier. This column size is commonly used for LC-MS applications.

Key Benefits

• RP Phenyl Hexyl™ stationary phase

• USP L11 equivalent chemistry

• Ultra-high purity silica support

• Fully double end-capped

• Six-carbon linker with terminal Phenyl group

• 100Å pore size

• Suitable for reversed-phase chromatography

• Enhanced π-π interactions

• Ideal for LC-MS applications

• Reduced solvent consumption with 2.1 mm ID format

Typical Applications

• LC-MS methods

• Reversed-phase HPLC

• USP L11 methods

• Aromatic compound separations

• Positional isomer separations

• Tocopherol analysis

• Flavonoid analysis

• Active pharmaceutical ingredient (API) analysis

• Natural product analysis

• Method development

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Phenyl Hexyl™

• Chromatography Mode: Reversed Phase

• USP Classification: L11

• Particle Size: 5 µm

• Pore Size: 100Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for reversed-phase chromatography

• USP L11 equivalent chemistry

• Fully double end-capped

• Enhanced π-π interactions improve aromatic selectivity

• Six-carbon linker increases distance from the silica surface

• Excellent for aromatic, polar, and structurally related compounds

• Often evaluated as an alternative to Restek™ Phenyl Hexyl columns in USP L11 methods

• Requires an HPLC system capable of reliable low-flow operation

• Well suited for LC-MS applications

• 150 mm length provides enhanced resolution for complex separations

• Methanol is often preferred over acetonitrile when maximizing π-π interactions

  ---