Catalog Number - 68539-15P

Cogent RP Phenyl Hexyl™ HPLC Columns are designed for reversed-phase separations where enhanced aromatic selectivity and strong π-π interactions are desired. Manufactured using ultra-high purity silica bonded with a six-carbon linker and terminal Phenyl group, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. RP Phenyl Hexyl™ is an excellent alternative for USP L11 methods that specify a Phenyl Hexyl stationary phase as well as for new method development.

68539-15P is packed with 5 µm RP Phenyl Hexyl™ particles in a 4.6 mm ID x 150 mm format with a 100Å pore size. The 5 µm particle size provides lower operating backpressure than smaller particles while maintaining excellent efficiency and reproducibility. The 150 mm column length offers enhanced chromatographic resolution compared to shorter columns, making it ideal for routine analytical methods, method development, and complex separations requiring improved resolving power.

The six-carbon linker positions the Phenyl group farther from the silica surface, increasing π-π interactions while reducing the influence of residual silanol activity. This enhanced selectivity can improve retention and separation of many aromatic and moderately polar analytes including positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. For optimized π-π interactions, methanol is often preferred over acetonitrile as the organic modifier. This is one of the most popular RP Phenyl Hexyl™ columns and is frequently selected as a first choice for USP monograph methods.

Key Benefits

• RP Phenyl Hexyl™ stationary phase

• USP L11 equivalent chemistry

• Ultra-high purity silica support

• Fully double end-capped

• Six-carbon linker with terminal Phenyl group

• 100Å pore size

• Suitable for reversed-phase chromatography

• Enhanced π-π interactions

• Alternative selectivity to conventional Phenyl phases

• Enhanced resolution for complex separations

Typical Applications

• Reversed-phase HPLC

• USP L11 methods

• Aromatic compound separations

• Positional isomer separations

• Tocopherol analysis

• Flavonoid analysis

• Polynuclear aromatic hydrocarbon analysis

• Nitro-aromatic compound separations

• Active pharmaceutical ingredient (API) analysis

• Natural product analysis

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Phenyl Hexyl™

• Chromatography Mode: Reversed Phase

• USP Classification: L11

• Particle Size: 5 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 150 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for reversed-phase chromatography

• USP L11 equivalent chemistry

• Fully double end-capped

• Enhanced π-π interactions improve aromatic selectivity

• Six-carbon linker increases distance from the silica surface

• Excellent for aromatic, polar, and structurally related compounds

• 150 mm length provides enhanced resolution for complex separations

• Methanol is often preferred over acetonitrile when maximizing π-π interactions

• Frequently selected for USP monograph methods

• One of the most popular RP Phenyl Hexyl™ column formats

• Compare performance to Restek Phenyl Hexyl