HPLC Column, RP Phenyl Hexyl, 5um, 2.1mm ID x 100mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl Hexyl™ HPLC Columns are designed for reversed-phase separations where enhanced aromatic selectivity and strong π-π interactions are desired. Manufactured using ultra-high purity silica bonded with a six-carbon linker and terminal Phenyl group, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. RP Phenyl Hexyl™ is an excellent alternative for certain USP L11 methods that specify or benefit from Phenyl Hexyl selectivity.
68539-10P-2 is packed with 5 µm RP Phenyl Hexyl™ particles in a 2.1 mm ID x 100 mm format with a 100Å pore size. The 5 µm particle size provides low operating backpressure and excellent reproducibility for LC-MS compatible methods. The 2.1 mm ID format reduces solvent consumption while improving mass spectrometry sensitivity. Typical operating flow rates are approximately 0.2–0.3 mL/min, and the HPLC system must be capable of delivering stable, accurate low-flow performance for optimum results.
The six-carbon linker positions the Phenyl group farther from the silica surface, increasing π-π interactions while reducing the influence of residual silanol activity. This enhanced selectivity can improve retention and separation of many aromatic and moderately polar analytes including positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. This column size is a favorite for LC-MS applications due to its balance of chromatographic efficiency, reduced solvent consumption, and mass spectrometry compatibility. For optimized π-π interactions, methanol is often preferred over acetonitrile as the organic modifier.
Key Benefits
RP Phenyl Hexyl™ stationary phase
USP L11 equivalent chemistry
Ultra-high purity silica support
Fully double end-capped
Six-carbon linker with terminal Phenyl group
100Å pore size
Suitable for reversed-phase chromatography
Enhanced π-π interactions
Ideal for LC-MS applications
Reduced solvent consumption with 2.1 mm ID format
Typical Applications
LC-MS methods
Reversed-phase HPLC
USP L11 methods
Aromatic compound separations
Positional isomer separations
Tocopherol analysis
Flavonoid analysis
Active pharmaceutical ingredient (API) analysis
Natural product analysis
Method development
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl Hexyl™
Chromatography Mode: Reversed Phase
USP Classification: L11
Particle Size: 5 µm
Pore Size: 100Å
Column Dimensions: 2.1 mm ID x 100 mm
Recommended Flow Rate: 0.2–0.3 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for reversed-phase chromatography
USP L11 equivalent chemistry
Fully double end-capped
Enhanced π-π interactions improve aromatic selectivity
Six-carbon linker increases distance from the silica surface
Excellent for aromatic, polar, and structurally related compounds
Requires an HPLC system capable of reliable low-flow operation
Well suited for LC-MS applications
100 mm length provides an excellent balance of resolution and analysis speed
Methanol is often preferred over acetonitrile when maximizing π-π interactions