Catalog Number - 68539-10P

Cogent RP Phenyl Hexyl™ HPLC Columns are designed for reversed-phase separations where enhanced aromatic selectivity and strong π-π interactions are desired. Manufactured using ultra-high purity silica bonded with a six-carbon linker and terminal Phenyl group, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. RP Phenyl Hexyl™ is an excellent alternative for certain USP L11 methods that specify or benefit from Phenyl Hexyl selectivity.

68539-10P is packed with 5 µm RP Phenyl Hexyl™ particles in a 4.6 mm ID x 100 mm format with a 100Å pore size. The 5 µm particle size provides lower operating backpressure than smaller particles while maintaining excellent efficiency and reproducibility. The 100 mm column length offers an excellent balance between chromatographic resolution and analysis speed, making it well suited for routine analytical methods, method development, and quality control applications.

The six-carbon linker positions the Phenyl group farther from the silica surface, increasing π-π interactions while reducing the influence of residual silanol activity. This enhanced selectivity can improve retention and separation of many aromatic and moderately polar analytes including positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. For optimized π-π interactions, methanol is often preferred over acetonitrile as the organic modifier.

Key Benefits

• RP Phenyl Hexyl™ stationary phase

• USP L11 equivalent chemistry

• Ultra-high purity silica support

• Fully double end-capped

• Six-carbon linker with terminal Phenyl group

• 100Å pore size

• Suitable for reversed-phase chromatography

• Enhanced π-π interactions

• Alternative selectivity to conventional Phenyl phases

• Excellent balance of speed and resolution

Typical Applications

• Reversed-phase HPLC

• USP L11 methods

• Aromatic compound separations

• Positional isomer separations

• Tocopherol analysis

• Flavonoid analysis

• Polynuclear aromatic hydrocarbon analysis

• Nitro-aromatic compound separations

• Active pharmaceutical ingredient (API) analysis

• Natural product analysis

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Phenyl Hexyl™

• Chromatography Mode: Reversed Phase

• USP Classification: L11

• Particle Size: 5 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for reversed-phase chromatography

• USP L11 equivalent chemistry

• Fully double end-capped

• Enhanced π-π interactions improve aromatic selectivity

• Six-carbon linker increases distance from the silica surface

• Excellent for aromatic, polar, and structurally related compounds

• 100 mm length provides an excellent balance of resolution and analysis speed

• Methanol is often preferred over acetonitrile when maximizing π-π interactions

• Well suited for method development and routine analytical testing