HPLC Column, RP Cyano, Wide Pore, 5um, 2.1mm ID x 100mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Cyano™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger polar and neutral compounds where alternative selectivity to traditional alkyl-bonded phases is desired. Manufactured using ultra-high purity silica bonded with a three-carbon ligand and terminal Cyano functionality, this stationary phase provides unique chromatographic characteristics that make it an excellent choice for USP L10 methods involving larger molecules and LC-MS applications. The 300Å wide pore structure improves analyte accessibility while maintaining the distinct selectivity of Cyano chemistry.
68534-10P-2-3M is packed with 5 µm RP Cyano™ Wide Pore particles in a 2.1 mm ID x 100 mm format with a 300Å pore size. The 5 µm particle size provides low operating backpressure and excellent reproducibility for LC-MS compatible methods. The 2.1 mm ID format reduces solvent consumption while improving mass spectrometry sensitivity. Typical flow rates are approximately 0.2–0.3 mL/min, and the HPLC system must be capable of delivering stable and accurate low-flow performance for optimum results.
The RP Cyano™ Wide Pore stationary phase is not end capped and is frequently used for proteins, peptides, enzymes, natural products, and other larger polar or neutral compounds in reversed-phase HPLC and LC-MS applications. Compared to non-modified silica columns, it provides shorter retention times and faster equilibration between runs. Packed and shipped in reversed-phase solvents. See Cogent NP Cyano Columns Packed in Normal-Phase Solvents for applications requiring normal-phase chromatography.
Key Benefits
RP Cyano™ Wide Pore stationary phase
USP L10 equivalent chemistry
Ultra-high purity silica support
Three-carbon linker with terminal Cyano group
Not end capped
300Å pore size
Suitable for reversed-phase chromatography
Ideal for LC-MS applications
Enhanced accessibility for larger molecules
Faster equilibration between runs
Typical Applications
LC-MS methods
Reversed-phase HPLC
USP L10 methods
Protein analysis
Peptide separations
Enzyme analysis
Natural product analysis
Polar compound separations
Method development
High-throughput laboratories
Specifications
Product Type: HPLC Column
Stationary Phase: RP Cyano™ Wide Pore
Chromatography Mode: Reversed Phase
USP Classification: L10
Particle Size: 5 µm
Pore Size: 300Å
Column Dimensions: 2.1 mm ID x 100 mm
Recommended Flow Rate: 0.2–0.3 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for reversed-phase chromatography
USP L10 equivalent chemistry
Not end capped
Designed for proteins, peptides, enzymes, and other larger molecules
Wide pore structure improves accessibility for large analytes
Ideal for LC-MS applications
Provides alternative selectivity compared to C18 and C8 columns
Faster equilibration than non-modified silica columns
Requires an HPLC system capable of reliable low-flow operation
Packed and shipped in reversed-phase solvents