HPLC Column, RP Cyano, Wide Pore, 5um, 4.6mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.

Additional Information:

Cogent RP Cyano™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger polar and neutral compounds where alternative selectivity to traditional alkyl-bonded phases is desired. Manufactured using ultra-high purity silica bonded with a three-carbon ligand and terminal Cyano functionality, this stationary phase provides unique chromatographic characteristics that make it an excellent choice for USP L10 methods involving larger molecules. The 300Å wide pore structure improves analyte accessibility while maintaining the distinct selectivity of Cyano chemistry.

68534-05P-3M is packed with 5 µm RP Cyano™ Wide Pore particles in a 4.6 mm ID x 50 mm format with a 300Å pore size. The 5 µm particle size provides lower operating backpressure than smaller particles while maintaining excellent efficiency and reproducibility. The short 50 mm column length is ideal for rapid analyses, high-throughput workflows, and method development applications where fast run times are important.

The RP Cyano™ Wide Pore stationary phase is not end capped and is frequently used for proteins, peptides, enzymes, natural products, and other larger polar or neutral compounds in reversed-phase HPLC. Compared to non-modified silica columns, it provides shorter retention times and faster equilibration between runs. Packed and shipped in reversed-phase solvents. See Cogent NP Cyano Columns Packed in Normal-Phase Solvents for applications requiring normal-phase chromatography.

Key Benefits

  • RP Cyano™ Wide Pore stationary phase

  • USP L10 equivalent chemistry

  • Ultra-high purity silica support

  • Three-carbon linker with terminal Cyano group

  • Not end capped

  • 300Å pore size

  • Suitable for reversed-phase chromatography

  • Enhanced accessibility for larger molecules

  • Faster equilibration between runs

  • Alternative selectivity to C18 and C8 columns

Typical Applications

  • Reversed-phase HPLC

  • USP L10 methods

  • Protein analysis

  • Peptide separations

  • Enzyme analysis

  • Natural product analysis

  • Polar compound separations

  • Neutral compound separations

  • Method development

  • High-throughput laboratories

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: RP Cyano™ Wide Pore

  • Chromatography Mode: Reversed Phase

  • USP Classification: L10

  • Particle Size: 5 µm

  • Pore Size: 300Å

  • Column Dimensions: 4.6 mm ID x 50 mm

  • Recommended Flow Rate: 1.0–2.0 mL/min

  • Column Hardware: Stainless Steel

  • Brand: Cogent

Important Notes

  • Suitable for reversed-phase chromatography

  • USP L10 equivalent chemistry

  • Not end capped

  • Designed for larger molecules including proteins, peptides, and enzymes

  • Wide pore structure improves accessibility for large analytes

  • Provides alternative selectivity compared to C18 and C8 columns

  • Faster equilibration than non-modified silica columns

  • Short 50 mm length helps reduce run times and solvent consumption

  • Packed and shipped in reversed-phase solvents

  • Normal-phase Cyano column alternatives are available


 

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