Catalog Number - 68518-SP-PG1-3

The 68518‑SP‑PG1‑3 Cogent RP C18 Wide Pore Guard Column Cartridges are designed to protect semi‑preparative HPLC columns used for large molecule separations, extending column life and maintaining chromatographic performance.

Packed with 5 µm Cogent RP C18™ Wide Pore stationary phase (300 Å), and manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, these cartridges are fully double end‑capped to provide:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible chromatographic protection

Constructed in stainless steel tubes with stainless steel frits, these guard cartridges are designed for durability in demanding semi‑prep applications.

Purpose of Guard Columns

• Protect columns from:

  • Particulate contamination

  • Strongly retained biomolecules

  • Matrix interferences

• Extend column lifetime

• Maintain consistent retention and peak shape

• Reduce downtime and replacement costs

300 Å Wide Pore – Biomolecule Protection

• Designed for large molecular weight analytes

• Ideal for:

  • Proteins

  • Peptides

  • Biomacromolecules

• Prevents pore blocking and restricted diffusion effects seen with standard 100 Å guard media

10 mm ID – Semi‑Preparative Compatibility

• Designed for 10 mm ID semi‑prep HPLC columns

• Supports higher sample loading workflows

• Maintains consistency in analytical‑to‑preparative scale‑up methods

⚠️ Requires a 10 mm ID prep column holder system (sold separately)

Why Choose Wide Pore Guard Cartridges (5 µm, 300 Å)

Matched Chemistry for Biomolecules

• Same wide‑pore C18 chemistry as the analytical/semi‑prep column

• Maintains method selectivity and retention behavior

• Prevents performance shifts

Enhanced Protection for Large Molecules

• Traps strongly retained biomolecules before reaching the column

• Reduces column fouling and degradation

Method Compatibility

• Suitable for USP L1 methods involving large molecules

• Supports biomolecule method development workflows

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Ensures stable biomolecule separations

High Purity Silica

• Consistent cartridge‑to‑cartridge performance

• Reliable protection across runs

Durable Construction

• Stainless steel housing and frits

  Designed for repeat use in semi‑prep systems

Typical Applications

• Semi‑preparative biomolecule HPLC

• Protein and peptide separations

• Method development and scale‑up workflows

• Pharmaceutical and QC applications

• Complex biological matrices

• Wide‑pore reversed‑phase methods (USP L1)

Important Usage Notes

• Designed for 300 Å wide‑pore (biomolecule) applications

• Must be used with a compatible guard column holder (10 mm ID)

• Replace regularly to maintain optimal column performance

• Critical for protecting high‑value semi‑prep columns

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.