Catalog Number - 68518-SP50

The 68518‑SP50 Cogent RP C18 Semi‑Prep HPLC Column is designed for semi‑preparative chromatography and purification workflows, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, high loading capacity, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak shape for polar and basic analytes

• Consistent and reproducible results across purification runs

50 mm Length – Fast Scouting & Purification

• Designed for short run times and rapid separations

• Ideal for method scouting prior to scale‑up

• Reduces solvent usage during development

• Enables high‑throughput purification workflows

10 mm ID – Semi‑Preparative Loading Capacity

• Provides significantly increased sample loading vs analytical columns

• Ideal for purification and isolation of compounds

• Supports semi‑preparative chromatography workflows

Typical Flow Rate Range

• ~2.0–5.0 mL/min (system dependent)

• Optimize flow based on instrument capability and method goals

✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and loading volumes

Flow Rate & System Considerations (10 mm ID, 5 µm)

• Requires HPLC systems capable of higher flow rates and increased loading capacity

• Compatible with:

  • Semi‑preparative pumps

  • Larger volume injection systems

  • مناسب fraction collection systems

⚠️ Not intended for standard low‑flow analytical HPLC systems

Why Choose RP C18 (5 µm, Semi‑Prep)

Scouting to Scale‑Up Platform

• Ideal for moving from analytical method development to semi‑prep purification

• Enables efficient method transfer and scale‑up workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids and similar analytes

Method Development & USP Compatibility

• Suitable for USP L1 methods

• Reliable choice for method development and purification workflows

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Reduces secondary interactions

• Improves peak symmetry

• Enhances performance for difficult analytes

High Purity Silica

• Consistent chromatographic results

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated purification cycles

• Reduced maintenance frequency

Typical Applications

• Semi‑preparative purification

• Method scouting and development

• Hydrophobic and neutral compound isolation

• Pharmaceutical and QC workflows

• Scale‑up from analytical HPLC

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for semi‑preparative HPLC systems (10 mm ID)

• Requires systems capable of higher flow rates and larger injection volumes

• Ideal for fast purification and method development workflows

• Optimize conditions for balance between resolution and throughput

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.