Semi Prep HPLC Column, RP C18 Wide Pore, 5um, 300A. 10mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑SP250‑3M Cogent RP C18 Wide Pore Semi‑Prep HPLC Column is engineered for maximum separation performance in biomolecule purification and method development, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and reproducible chromatographic results.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and reliable purification performance
The 300 Å pore size allows large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is ideal as a USP L1 alternate for large molecule methods and as a high‑resolution platform for semi‑prep purification and scale‑up workflows.
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the 10 mm wide‑pore C18 range
Maximizes peak capacity for complex biomolecule mixtures
Ideal for proteins, peptides, and closely related macromolecules
Provides extended retention and improved separation of difficult analytes
Best suited for final purification and highest‑purity isolation
This format is selected when shorter columns cannot achieve required resolution or purification performance.
10 mm ID – Semi‑Preparative Loading Capacity
Provides significantly increased loading capacity vs analytical columns
Ideal for biomolecule purification and compound isolation
Supports semi‑preparative chromatography workflows
Typical Flow Rate Range
~2.0–5.0 mL/min (system dependent)
Optimize based on instrument capability and purification goals
✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and larger injection volumes
Flow Rate & System Considerations (10 mm ID, 5 µm, 300 Å)
Requires systems capable of:
Higher flow rates
Large injection volumes
Compatible with:
Semi‑prep pumps
Fraction collection systems
High‑volume injection systems
⚠️ Not intended for standard analytical HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Semi‑Prep)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
High‑Resolution Purification Platform
Ideal for final-stage purification workflows
Maximizes separation performance and product purity
Supports complex biomolecule separations
Scouting to Scale‑Up Workflow
Predictable selectivity for transition to larger preparative columns
Supports efficient analytical‑to‑semi‑prep and scale‑up workflows
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs
Durable & Cost‑Efficient
Long column lifetime
Ideal for repeated purification cycles
Lower maintenance requirements
Typical Applications
Semi‑preparative biomolecule purification
Protein and peptide separations
High‑purity compound isolation
Complex biomolecule mixtures
Pharmaceutical and QC workflows
Analytical to preparative scale‑up
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires semi‑prep HPLC system capability (10 mm ID)
Ideal for maximum‑resolution purification workflows
Optimize conditions for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable