Catalog Number - 68518-SP250

The 68518‑SP250 Cogent RP C18 Semi‑Prep HPLC Column is designed for maximum separation performance in purification and method development workflows, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, high loading capacity, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak symmetry for polar and basic analytes

• Consistent and reproducible purification results

This column is ideal as a USP L1 alternate and as a high‑resolution platform for semi‑prep purification and scale‑up workflows.

250 mm Length – Maximum Separation & Peak Capacity

• Highest resolving power in the 10 mm semi‑prep RP C18 range

• Maximizes peak capacity for highly complex mixtures

• Ideal for closely related compounds and challenging separations

• Provides extended retention for hydrophobic analytes

• Best suited for final purification and highest purity isolation

This format is selected when shorter columns cannot achieve required resolution or purification performance.

10 mm ID – Semi‑Preparative Loading Capacity

• Provides significantly increased loading capacity vs analytical columns

• Ideal for compound isolation and purification workflows

• Supports semi‑preparative chromatography applications

Typical Flow Rate Range

• ~2.0–5.0 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and injection volumes

Flow Rate & System Considerations (10 mm ID, 5 µm)

• Requires systems capable of:

  • Higher flow rates

  • Large injection volumes

• Compatible with:

  • Semi‑prep pumps

  • Fraction collection systems

  • High‑volume injection systems

⚠️ Not intended for standard analytical HPLC systems

Why Choose RP C18 (5 µm, Semi‑Prep)

Maximum Resolution for Purification

• Ideal for final purification of complex mixtures

• Maximizes separation performance and product purity

• Supports high‑purity isolation workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids

• Provides greater retention than C8 phases

Method Development & USP Compatibility

• Suitable for USP L1 methods

• Reliable platform for method development and validation workflows

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for challenging analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability across runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated purification cycles

• Reduced maintenance frequency

Typical Applications

• Semi‑preparative purification

• High‑purity compound isolation

• Complex mixture separations

• Pharmaceutical and QC workflows

• Analytical to preparative scale‑up

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for semi‑preparative HPLC systems (10 mm ID)

• Requires systems capable of higher flow rates and loading volumes

• Ideal for maximum‑resolution purification workflows

• Optimize for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.