Catalog Number - 68518-SP150-3M

The 68518‑SP150‑3M Cogent RP C18 Wide Pore Semi‑Prep HPLC Column is designed for high‑resolution biomolecule purification and method development, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible purification results

The 300 Å pore size allows large molecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.

This column is ideal as a USP L1 alternate for large molecule applications and as a platform for semi‑prep purification and scale‑up workflows.

150 mm Length – Maximum Resolution for Biomolecule Purification

• Higher resolving power than 50 mm and 100 mm columns

• Maximizes peak capacity for complex biomolecule mixtures

• Ideal for proteins, peptides, and closely related macromolecules

• Preferred for final purification and method refinement

• Longer run times compared to shorter column formats

This format is selected when maximum separation performance is required for purification and isolation.

10 mm ID – Semi‑Preparative Loading Capacity

• Provides significantly increased loading capacity vs analytical columns

• Ideal for biomolecule purification and isolation

• Supports semi‑preparative chromatography workflows

Typical Flow Rate Range

• ~2.0–5.0 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and larger injection volumes

Flow Rate & System Considerations (10 mm ID, 5 µm, 300 Å)

• Requires systems capable of:

  • Higher flow rates

  • Large injection volumes

• Compatible with:

  • Semi‑prep pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for standard analytical HPLC systems

Why Choose Wide Pore (300 Å) C18 (5 µm, Semi‑Prep)

Designed for Biomolecules

• Enables purification of:

  • Proteins

  • Peptides

  • Large hydrophobic compounds

• Eliminates mass transfer limitations of smaller pore materials

Method Development to Scale‑Up

• Ideal for developing and refining biomolecule purification methods

• Predictable selectivity for transition to larger preparative columns

• Supports efficient analytical‑to‑semi‑prep workflows

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for biomolecule separations

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated purification cycles

• Lower maintenance requirements

Typical Applications

• Semi‑preparative biomolecule purification

• Protein and peptide separations

• Method development and optimization

• Hydrophobic and neutral compound isolation

• Analytical to preparative scale‑up workflows

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Requires semi‑prep HPLC system capability (10 mm ID)

• Ideal for maximum‑resolution purification workflows

• Optimize for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.