Catalog Number - 68518-SP150

The 68518‑SP150 Cogent RP C18 Semi‑Prep HPLC Column is engineered for high‑resolution purification and method development, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, high loading capacity, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak symmetry for polar and basic analytes

• Consistent and reproducible purification results

This column is ideal as a USP L1 alternate and a robust platform for semi‑preparative method development and purification workflows.

150 mm Length – Maximum Resolution for Semi‑Prep Purification

• Higher resolving power than 50 mm and 100 mm columns

• Maximizes peak capacity for complex mixtures

• Ideal for closely related compounds and difficult separations

• Preferred for final method refinement prior to full preparative scale‑up

• Longer run times compared to shorter formats

This configuration is selected when maximum separation performance is required for purification and isolation.

10 mm ID – Semi‑Preparative Loading Capacity

• Provides significantly increased loading capacity vs analytical columns

• Ideal for compound isolation and purification

• Supports semi‑preparative chromatography workflows

Typical Flow Rate Range

• ~2.0–5.0 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and injection volumes

Flow Rate & System Considerations (10 mm ID, 5 µm)

• Requires systems capable of higher flow rates and larger injection volumes

• Compatible with:

  • Semi‑prep pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for standard low‑flow analytical HPLC systems

Why Choose RP C18 (5 µm, Semi‑Prep)

Method Development to Scale‑Up

• Ideal for moving from analytical separations to purification workflows

• Enables efficient method transfer and process development

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids and similar analytes

Method Compatibility

• Suitable for USP L1 methods

• Reliable platform for purification and analytical workflows

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for challenging analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated purification cycles

• Lower maintenance requirements

Typical Applications

• Semi‑preparative purification

• Method development and optimization

• Hydrophobic and neutral compound isolation

• Pharmaceutical and QC workflows

• Analytical to preparative scale‑up

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for semi‑preparative HPLC systems (10 mm ID)

• Requires systems capable of higher flow rates and loading volumes

• Ideal for maximum‑resolution purification workflows

• Optimize for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.