Catalog Number - 68518-SP100-3M

The 68518‑SP100‑3M Cogent RP C18 Wide Pore Semi‑Prep HPLC Column is designed for biomolecule purification and method development, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible purification performance

The 300 Å pore size enables large molecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.

This column is ideal as a USP L1 alternate for large molecule applications and as a development platform for semi‑prep purification workflows.

100 mm Length – Increased Resolution for Biomolecule Purification

• Higher resolving power than 50 mm scouting columns

• Improved separation of proteins, peptides, and complex biomolecules

• Increased peak capacity for purification workflows

• Ideal for refining separations prior to preparative scale‑up

This format is selected when additional resolution is required beyond initial scouting runs.

10 mm ID – Semi‑Preparative Loading Capacity

• Provides significantly increased loading capacity vs analytical columns

• Ideal for purification and isolation of biomolecules

• Supports semi‑preparative chromatography workflows

Typical Flow Rate Range

• ~2.0–5.0 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and injection volumes

Flow Rate & System Considerations (10 mm ID, 5 µm, 300 Å)

• Requires systems capable of higher flow rates and larger injection volumes

• Compatible with:

  • Semi‑prep pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not suitable for standard low‑flow analytical HPLC systems

Why Choose Wide Pore (300 Å) C18 (5 µm, Semi‑Prep)

Designed for Biomolecules

• Enables purification of:

  • Proteins

  • Peptides

  • Large hydrophobic compounds

• Eliminates mass transfer limitations of smaller pore materials

Method Development to Scale‑Up

• Ideal for developing and optimizing biomolecule purification methods

• Predictable selectivity when transitioning to larger preparative columns

• Supports efficient analytical‑to‑semi‑prep workflows

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Stabilizes biomolecule separations

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated purification cycles

• Lower maintenance frequency

Typical Applications

• Semi‑preparative biomolecule purification

• Protein and peptide separations

• Method development and optimization

• Hydrophobic and neutral compound isolation

• Analytical to preparative scale‑up workflows

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Requires semi‑prep HPLC system capability (10 mm ID)

• Ideal for refining purification methods prior to scale‑up

• Optimize for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This product is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

Please contact Customer Service for formal quotation, lead time, and ordering assistance.