Catalog Number - 68518-SP100

The 68518‑SP100 Cogent RP C18 Semi‑Prep HPLC Column is designed for method development and purification workflows, combining 5 µm particles with fully double end‑capped C18 bonding to deliver reliable retention, increased sample loading capacity, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak symmetry for polar and basic analytes

• Consistent and reproducible purification performance

100 mm Length – Increased Resolution for Purification

• Higher resolving power compared to 50 mm scouting columns

• Improved separation of closely related compounds

• Increased peak capacity for purification workflows

• Ideal for refining separations prior to larger‑scale prep

This format is selected when additional resolution is needed beyond initial scouting while maintaining efficient run times.

10 mm ID – Semi‑Preparative Loading Capacity

• Provides significantly higher loading capacity than analytical columns

• Ideal for compound isolation and purification

• Supports semi‑preparative chromatography workflows

Typical Flow Rate Range

• ~2.0–5.0 mL/min (system dependent)

• Optimize based on instrument capability and separation goals

✅ Designed for semi‑preparative HPLC systems capable of higher flow and injection volumes

Flow Rate & System Considerations (10 mm ID, 5 µm)

• Requires HPLC systems capable of higher flow rates and larger injection volumes

• Compatible with:

  • Semi‑prep pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for standard low‑flow analytical HPLC systems

Why Choose RP C18 (5 µm, Semi‑Prep)

Method Development to Scale‑Up

• Ideal for moving from analytical methods to semi‑prep purification

• Enables efficient method transfer and process development workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids and similar analytes

Method Compatibility

• Suitable for USP L1 methods

• Reliable for routine purification and analytical workflows

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for challenging analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability across runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated purification cycles

• Reduced maintenance frequency

Typical Applications

• Semi‑preparative purification

• Method development and optimization

• Hydrophobic and neutral compound isolation

• Pharmaceutical and QC workflows

• Scale‑up from analytical HPLC

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for semi‑preparative HPLC systems (10 mm ID)

• Requires systems capable of higher flow rates and loading volumes

• Ideal for refining purification methods prior to scale‑up

• Optimize for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.