Process HPLC Column, RP C18, Wide Pore, 5um, 300A. 50mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑P50‑2503M Cogent RP C18 Wide Pore Process HPLC Column is engineered for maximum‑resolution biomolecule purification in industrial and manufacturing environments, combining 5 µm particles with a 300 Å wide‑pore structure to deliver optimal analyte accessibility, extremely high loading capacity, and consistent chromatographic performance at process scale.
Manufactured using high‑purity 300 Å silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Reliable and reproducible purification performance across large‑batch and continuous processing workflows
The 300 Å pore structure enables large biomolecules to fully penetrate the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is ideal as a USP L1 alternate for large‑molecule applications and serves as a final‑stage platform for high‑purity biomolecule manufacturing and industrial process chromatography.
250 mm Length – Maximum Separation & Highest Purity
Highest resolving power in the 50 mm wide‑pore C18 process range
Maximizes peak capacity for highly complex biomolecule mixtures
Ideal for proteins, peptides, and closely related macromolecules
Provides extended retention for difficult and hydrophobic analytes
Best suited for final purification and highest‑purity isolation in process workflows
This configuration is selected when no shorter column can meet resolution or purity requirements at industrial scale.
50 mm ID – Maximum Industrial‑Scale Loading
Provides extremely high sample loading capacity (top tier across entire column system)
Designed for industrial purification, batch manufacturing, and continuous processing workflows
Supports ultra‑high‑flow chromatography systems
Typical Flow Rate Range
~50–150 mL/min (system dependent)
Optimize based on process requirements and production demands
✅ Designed for process HPLC systems capable of ultra‑high flow rates and very large injection volumes
Flow Rate & System Considerations (50 mm ID, 5 µm, 300 Å)
Requires systems capable of:
Ultra‑high flow rates
Very large injection volumes
Compatible with:
Process chromatography systems
Industrial pumps and injection platforms
Large‑volume fraction collection or continuous processing systems
⚠️ Not suitable for analytical, semi‑prep, or standard preparative HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Process Scale)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations associated with smaller pore media
Maximum‑Resolution Industrial Platform
Ideal for final‑stage purification at manufacturing scale
Maximizes separation efficiency, recovery, and product purity
Supports high‑purity biomolecule production workflows
Full Workflow Scalability
Maintains consistent selectivity across analytical → semi‑prep → prep → production → process
Enables efficient method transfer and industrial scale‑up
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations at industrial scale
High Purity Silica
Consistent chromatographic performance
Reduced variability across large‑batch and continuous runs
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load, continuous process environments
Reduces downtime and operational cost
Typical Applications
Industrial‑scale biomolecule purification
Protein and peptide separations
High‑purity compound manufacturing
Pharmaceutical production
Process chromatography and scale‑up
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires process‑scale HPLC system capability (50 mm ID)
Ideal for maximum‑resolution, high‑purity industrial workflows
Optimize for resolution vs throughput based on production objectives
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable