Process HPLC Column, RP C18, Wide Pore, 5um, 300A. 50mm ID x 150mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑P50‑1503M Cogent RP C18 Wide Pore Process HPLC Column is engineered for high‑resolution biomolecule purification and industrial‑scale process chromatography, combining 5 µm particles with a 300 Å wide‑pore structure to deliver maximum analyte accessibility, extremely high loading capacity, and consistent large‑scale chromatographic performance.
Manufactured using high‑purity 300 Å silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Reliable and reproducible purification performance in high‑throughput process environments
The 300 Å pore structure enables large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is ideal as a USP L1 alternate for large‑molecule methods and as a final‑stage platform for industrial biomolecule purification workflows.
150 mm Length – Maximum Resolution for Process‑Scale Purification
Higher resolving power than 50 mm and 100 mm process columns
Maximizes peak capacity for complex biomolecule mixtures
Ideal for proteins, peptides, and closely related macromolecules
Preferred for final purification and process optimization before manufacturing deployment
Longer run times compared to shorter formats
This configuration is selected when maximum separation performance is required prior to full‑scale production or continuous processing.
50 mm ID – Industrial‑Scale Loading Capacity
Provides extremely high sample loading capacity (beyond preparative scale)
Designed for industrial purification and manufacturing workflows
Supports ultra‑high‑flow chromatography systems
Typical Flow Rate Range
~50–150 mL/min (system dependent)
Optimize based on process requirements and system capability
✅ Designed for process HPLC systems with ultra‑high flow rates and very large injection volumes
Flow Rate & System Considerations (50 mm ID, 5 µm, 300 Å)
Requires systems capable of:
Ultra‑high flow rates
Very large injection volumes
Compatible with:
Process chromatography systems
Industrial pumps and injection platforms
Large‑volume fraction collection or continuous processing systems
⚠️ Not suitable for analytical, semi‑prep, or standard preparative HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Process Scale)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
Maximum‑Resolution Industrial Platform
Ideal for final-stage biomolecule purification
Maximizes separation efficiency and product purity
Supports high‑purity manufacturing and process chromatography workflows
Scalable Workflow Integration
Predictable selectivity across analytical → semi‑prep → prep → production → process
Enables efficient method transfer and industrial scale‑up
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations at scale
High Purity Silica
Consistent chromatographic performance
Reduced variability across large‑batch and continuous process runs
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load and continuous process workflows
Reduces downtime and operational cost
Typical Applications
Process‑scale biomolecule purification
Protein and peptide separations
Industrial pharmaceutical manufacturing
High‑purity compound isolation
Process development and optimization
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires process‑scale HPLC system capability (50 mm ID)
Ideal for maximum‑resolution purification prior to full‑scale production runs
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable