Process HPLC Column, RP C18, Wide Pore, 5um, 300A. 50mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑P50‑100‑3M Cogent RP C18 Wide Pore Process HPLC Column is designed for industrial‑scale biomolecule purification and process chromatography development, combining 5 µm particles with a 300 Å wide‑pore structure to deliver maximum analyte accessibility, extremely high loading capacity, and consistent chromatographic performance.
Manufactured using high‑purity 300 Å silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Reliable and reproducible purification performance at process scale
The 300 Å pore structure allows large biomolecules to fully penetrate the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is ideal as a USP L1 alternate for large‑molecule methods and as a refinement platform for industrial‑scale biomolecule purification workflows.
100 mm Length – Increased Resolution for Process Development
Higher resolving power than 50 mm process scouting columns
Improved separation of proteins, peptides, and complex biomolecules
Increased peak capacity for large‑scale purification workflows
Ideal for refining methods prior to extended production runs or longer columns
This configuration is selected when additional resolution is required beyond initial process‑scale screening.
50 mm ID – Industrial‑Scale Loading Capacity
Provides extremely high sample loading capacity (beyond preparative scale)
Designed for industrial purification and manufacturing workflows
Supports ultra‑high‑flow chromatography systems
Typical Flow Rate Range
~50–150 mL/min (system dependent)
Optimize based on process requirements and system capability
✅ Designed for process HPLC systems with ultra‑high flow rates and very large injection volumes
Flow Rate & System Considerations (50 mm ID, 5 µm, 300 Å)
Requires systems capable of:
Ultra‑high flow rates
Very large injection volumes
Compatible with:
Process chromatography systems
Industrial pumps and injection platforms
Large‑volume fraction collection or continuous processing systems
⚠️ Not suitable for analytical, semi‑prep, or standard preparative HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Process Scale)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
Process‑Scale Biomolecule Purification
Ideal for large‑scale and industrial workflows
Maximizes throughput and recovery of large molecules
Supports high‑purity biomolecule manufacturing
Scalable Workflow Integration
Predictable selectivity across analytical → semi‑prep → prep → production → process
Supports efficient method transfer and process scale‑up
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations at scale
High Purity Silica
Consistent chromatographic performance
Reduced variability across large‑batch and continuous runs
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load and continuous process workflows
Reduced downtime and operational cost
Typical Applications
Process‑scale biomolecule purification
Protein and peptide separations
Industrial pharmaceutical manufacturing
Process development and scale‑up
Large‑scale compound isolation
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires process‑scale HPLC system capability (50 mm ID)
Ideal for method refinement prior to full‑production or continuous processes
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable