Catalog Number - 68518-P30-PG1-3

The 68518‑P30‑PG1‑3 Cogent RP C18 Wide Pore Prep Guard Column Cartridge is engineered to protect high‑value preparative columns in large‑scale biomolecule purification workflows, ensuring extended column life and consistent chromatographic performance.

Packed with 5 µm Cogent RP C18™ Wide Pore stationary phase (300 Å) and manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this cartridge is fully double end‑capped to provide:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible chromatographic protection

Constructed in a stainless steel housing with stainless steel frits, this cartridge is designed for durability in high‑flow, high‑load production environments.

Purpose of Guard Columns

• Protect preparative columns from:

  • Particulate contamination

  • Strongly retained biomolecules

  • Matrix buildup and fouling

• Extend column lifetime

• Maintain consistent retention and peak shape

• Reduce downtime and replacement costs

300 Å Wide Pore – Biomolecule Protection

• Designed specifically for large molecular weight analytes

• Ideal for:

  • Proteins

  • Peptides

  • Macromolecules

• Prevents:

  • Restricted diffusion effects

  • Pore blocking common with 100 Å media

5 µm Guard Media – Analytical to Production Consistency

• Matches 5 µm column packing used in analytical, semi‑prep, and preparative workflows

• Maintains consistent selectivity and retention behavior

• Ideal for method transfer and scale‑up consistency

✅ Ensures method integrity across full workflow (analytical → semi‑prep → prep → production)

30 mm ID – Production‑Scale Systems

• Designed for 30 mm ID preparative HPLC columns

• Supports very high flow rates and large injection volumes

• Ideal for pilot‑scale and full production purification workflows

⚠️ Requires use with a 30 mm ID prep guard column holder system

Why Choose Wide Pore Guard Cartridges (5 µm, 300 Å, 30 mm)

Matched Chemistry for Biomolecules

• Same wide‑pore C18 chemistry as the primary column

• Maintains selectivity and chromatographic behavior

• Prevents method disruption

Enhanced Biomolecule Protection

• Captures strongly retained large molecules before reaching the column

• Reduces column fouling and degradation

Production‑Scale Alignment

• Designed for high‑load purification and manufacturing workflows

• Supports consistent scale‑up performance

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Stabilizes performance for biomolecule separations

High Purity Silica

• Reliable cartridge‑to‑cartridge reproducibility

• Consistent protection across extended runs

Durable Construction

• Stainless steel housing and frits

• Designed for high‑flow production systems

Typical Applications

• Production‑scale biomolecule purification

• Protein and peptide separations

• Preparative column protection

• Method development and scale‑up

• Pharmaceutical manufacturing and QC

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Must be used with a compatible 30 mm prep guard column holder

• Replace regularly to maintain optimal column performance

• Essential for protecting high‑value production columns

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.