Prep HPLC Column, RP C18, Wide Pore, 5um, 300A. 30mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑P30‑250‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is engineered for maximum‑resolution biomolecule purification and production‑scale separations, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, extremely high loading capacity, and consistent chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Reliable and reproducible purification performance in high‑throughput environments
The 300 Å pore size allows large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is widely used in pilot plants and full production environments as a USP L1 alternate for large‑molecule separations.
250 mm Length – Maximum Separation & Highest Purity
Highest resolving power in the 30 mm wide‑pore C18 range
Maximizes peak capacity for highly complex biomolecule mixtures
Ideal for proteins, peptides, and closely related macromolecules
Provides extended retention and improved separation of difficult analytes
Best suited for final purification and highest‑purity isolation
This configuration is selected when shorter columns cannot meet resolution or purity requirements at production scale.
30 mm ID – Maximum Production‑Scale Loading
Provides extremely high sample loading capacity
Designed for large‑scale biomolecule purification and manufacturing workflows
Supports very high‑flow preparative chromatography systems
Typical Flow Rate Range
~20–60 mL/min (system dependent)
Optimize based on instrument capability and production requirements
✅ Designed for production‑scale HPLC systems with high flow rates and large injection volumes
Flow Rate & System Considerations (30 mm ID, 5 µm, 300 Å)
Requires systems capable of:
Very high flow rates
Large injection volumes
Compatible with:
Production preparative pumps
Large‑volume injection systems
High‑capacity fraction collection systems
⚠️ Not suitable for analytical or semi‑prep HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Production Scale)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
Maximum‑Resolution Production Platform
Ideal for final‑stage purification workflows
Maximizes separation efficiency and product purity
Supports high‑purity biomolecule manufacturing
Scalable Workflow Integration
Predictable selectivity across analytical → semi‑prep → prep → production
Enables efficient method transfer and scale‑up
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability across runs and production batches
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load production workflows
Reduced downtime and replacement costs
Typical Applications
Production‑scale biomolecule purification
Protein and peptide separations
High‑purity compound isolation
Pharmaceutical manufacturing and QC
Process development and industrial scale‑up
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires production‑scale HPLC system capability (30 mm ID)
Ideal for maximum‑resolution, high‑purity production workflows
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable