Catalog Number - 68518-P21-PG1-3

The 68518‑P21‑PG1‑3 Cogent RP C18 Wide Pore Prep Guard Column Cartridge is designed to protect preparative HPLC columns used for biomolecule separations, extending column life while maintaining consistent chromatographic performance.

Packed with 5 µm Cogent RP C18™ Wide Pore stationary phase (300 Å) and manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this cartridge is fully double end‑capped to provide:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible chromatographic protection

Constructed in a stainless steel housing with stainless steel frits, this guard cartridge ensures durability in high‑flow, high‑load preparative environments.

Purpose of Guard Columns

• Protect preparative columns from:

  • Particulate contamination

  • Strongly retained biomolecules

  • Sample matrix buildup and fouling

• Extend column lifetime

• Maintain consistent retention and peak shape

• Reduce downtime and replacement costs

300 Å Wide Pore – Biomolecule Protection

• Designed specifically for large molecular weight analytes

• Ideal for:

  • Proteins

  • Peptides

  • Macromolecules

• Prevents:

  • Restricted diffusion effects

  • Pore blockage seen with 100 Å materials

5 µm Guard Media – Analytical to Prep Compatibility

• Matches 5 µm analytical and preparative column packing

• Maintains consistent selectivity and retention behavior

• Ideal for method transfer from analytical to preparative scale

✅ Ensures method integrity across workflows

21.2 mm ID – Preparative Scale Systems

• Designed for 21.2 mm ID preparative HPLC columns

• Supports high‑flow, high‑load purification workflows

• Maintains performance for large‑scale separations

⚠️ Requires use with a 21.2 mm ID prep guard column holder system

Why Choose Wide Pore Guard Cartridges (5 µm, 300 Å)

Matched Chemistry for Biomolecules

• Same wide‑pore C18 chemistry as the main column

• Maintains selectivity and separation behavior

• Prevents unwanted chromatographic shifts

Enhanced Biomolecule Protection

• Captures strongly retained large molecules before reaching the column

• Reduces column fouling and degradation

Preparative Workflow Alignment

• Designed for biomolecule purification and large‑scale workflows

• Supports analytical → semi‑prep → preparative consistency

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances stability for sensitive biomolecule separations

High Purity Silica

• Consistent cartridge performance

• Reliable protection across runs

Durable Construction

• Stainless steel housing and frits

• Designed for high‑flow preparative systems

Typical Applications

• Preparative biomolecule purification

• Protein and peptide separations

• Column protection in high‑load workflows

• Method development and scale‑up

• Pharmaceutical and QC applications

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Must be used with a compatible 21.2 mm prep guard holder

• Replace regularly to maintain optimal column performance

• Essential for protecting high‑value preparative columns

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.