Catalog Number - 68518-P21-50-3M

The 68518‑P21‑50‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is designed for high‑capacity biomolecule purification and preparative method development, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and reliable chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible purification performance

The 300 Å pore size enables large molecules to fully penetrate the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.

This column is widely used in laboratories and pilot‑scale operations as a USP L1 alternate for large‑molecule separations.

50 mm Length – Fast Biomolecule Scouting & Purification

• Designed for rapid separations and short run times

• Ideal for early‑stage preparative method development

• Minimizes solvent consumption during screening

• Enables high‑throughput purification workflows

21.2 mm ID – High‑Capacity Preparative Scale

• Provides very high loading capacity for large‑scale purification

• Ideal for protein, peptide, and biomolecule isolation

• Supports preparative chromatography workflows

Typical Flow Rate Range

• ~10–30 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes

Flow Rate & System Considerations (21.2 mm ID, 5 µm, 300 Å)

• Requires systems capable of:

  • High flow rates

  • Large injection volumes

• Compatible with:

  • Preparative pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for analytical or semi‑prep HPLC systems

Why Choose Wide Pore (300 Å) C18 (5 µm, Preparative)

Designed for Biomolecules

• Enables purification of:

  • Proteins

  • Peptides

  • Large hydrophobic compounds

• Eliminates mass transfer limitations of smaller pore materials

Ideal for Preparative Scouting

• Used to develop and optimize biomolecule purification methods

• Predictable selectivity for transition to longer columns or larger scale systems

• Supports efficient method scaling workflows

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances stability for biomolecule separations

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs and batches

Durable & Cost‑Efficient

• Long column lifetime

• Designed for high‑load purification workflows

• Reduced maintenance requirements

Typical Applications

• Preparative biomolecule purification

• Protein and peptide separations

• Pilot‑scale purification and method development

• Complex biomolecule mixtures

• Pharmaceutical and QC processes

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Requires preparative HPLC system capability (21.2 mm ID)

• Ideal for fast screening prior to larger‑scale purification

• Optimize for throughput vs resolution balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.