Catalog Number - 68518-P21-50

The 68518‑P21‑50 Cogent RP C18 Preparative HPLC Column is designed for high‑capacity purification and preparative method development, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, excellent durability, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak shape for polar and basic analytes

• Consistent and reproducible purification results

This column is ideal as a USP L1 alternate and a foundation for preparative‑scale method development and purification workflows.

50 mm Length – Fast Preparative Scouting

• Designed for rapid separations and short run times

• Ideal for early‑stage preparative method development

• Minimizes solvent use during screening

• Enables high‑throughput purification workflows

21.2 mm ID – Preparative Loading Capacity

• Provides very high sample loading capacity

• Designed for large‑scale purification and isolation

• Supports preparative chromatography workflows

Typical Flow Rate Range

• ~10–30 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes

Flow Rate & System Considerations (21.2 mm ID, 5 µm)

• Requires systems capable of:

  • High flow rates

  • Large injection volumes

• Compatible with:

  • Preparative pumps

  • Large‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for standard analytical or semi‑prep HPLC systems

Why Choose RP C18 (5 µm, Preparative Scale)

Ideal for Preparative Scouting

• Used to develop and optimize purification methods before scaling further

• Enables rapid method screening and adjustment

• Supports efficient scale‑up workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids

• Provides greater retention than C8 phases

Method Development & USP Compatibility

• Suitable for USP L1 methods

• Reliable replacement or alternate column

• Supports regulated and validated workflows

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for challenging analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs and batches

Durable & Cost‑Efficient

• Long column lifetime

• Designed for high‑load purification cycles

• Reduced maintenance requirements

Typical Applications

• Preparative purification

• Method development and optimization

• Hydrophobic and neutral compound isolation

• Pharmaceutical and QC processes

• High‑load separations

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for preparative HPLC systems (21.2 mm ID)

• Requires systems capable of high flow rates and large loading volumes

• Ideal for fast preparative screening workflows

• Optimize conditions for throughput vs resolution balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.