Prep HPLC Column, RP C18, Wide Pore, 5um, 300A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑P21‑250‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is engineered for maximum‑resolution biomolecule purification and production‑scale separations, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and consistent chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Reliable and reproducible purification performance
The 300 Å pore size allows large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is widely used in pilot‑scale and production‑level purification environments as a USP L1 alternate for large‑molecule separations.
250 mm Length – Maximum Separation & Highest Purity
Highest resolving power in the 21.2 mm wide‑pore C18 range
Maximizes peak capacity for highly complex biomolecule mixtures
Ideal for proteins, peptides, and closely related macromolecules
Provides extended retention and improved separation of difficult analytes
Best suited for final purification and highest‑purity isolation
This configuration is selected when shorter columns cannot achieve required resolution or purity.
21.2 mm ID – Production‑Scale Loading Capacity
Provides very high sample loading capacity
Designed for large‑scale biomolecule purification and batch processing
Supports high‑flow preparative chromatography workflows
Typical Flow Rate Range
~10–30 mL/min (system dependent)
Optimize based on instrument capability and purification requirements
✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes
Flow Rate & System Considerations (21.2 mm ID, 5 µm, 300 Å)
Requires systems capable of:
High flow rates
Large injection volumes
Compatible with:
Preparative pumps
High‑volume injection systems
Fraction collection systems
⚠️ Not intended for analytical or semi‑prep HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Preparative)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
Maximum‑Resolution Purification Platform
Ideal for final‑stage purification workflows
Maximizes separation efficiency and product purity
Supports complex biomolecule separations
Scalable Method Development
Predictable selectivity when transitioning from semi‑prep to full production scale
Supports efficient analytical → semi‑prep → preparative workflows
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs and batches
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load purification cycles
Reduced maintenance requirements
Typical Applications
Production‑scale biomolecule purification
Protein and peptide separations
High‑purity compound isolation
Large‑scale process development
Pharmaceutical and QC workflows
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires preparative HPLC system capability (21.2 mm ID)
Ideal for maximum‑resolution, high‑purity workflows
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable