Prep HPLC Column, RP C18, Wide Pore, 5um, 300A. 21.2mm ID x 150mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑P21‑150‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is engineered for high‑resolution biomolecule purification and preparative method development, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and reliable chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and reproducible purification results
The 300 Å pore size enables large biomolecules to fully penetrate the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is widely used in laboratories and pilot‑scale purification environments as a USP L1 alternate for large‑molecule separations.
150 mm Length – Maximum Resolution for Biomolecule Purification
Higher resolving power than 50 mm and 100 mm columns
Maximizes peak capacity for complex biomolecule mixtures
Ideal for proteins, peptides, and closely related macromolecules
Preferred for final purification and method refinement
Longer run times compared to shorter column formats
This configuration is selected when maximum separation performance is required prior to production‑scale purification.
21.2 mm ID – High‑Capacity Preparative Scale
Provides very high sample loading capacity
Designed for large‑scale biomolecule purification
Supports high‑flow preparative chromatography workflows
Typical Flow Rate Range
~10–30 mL/min (system dependent)
Optimize based on instrument capability and purification requirements
✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes
Flow Rate & System Considerations (21.2 mm ID, 5 µm, 300 Å)
Requires systems capable of:
High flow rates
Large injection volumes
Compatible with:
Preparative pumps
High‑volume injection systems
Fraction collection systems
⚠️ Not intended for analytical or semi‑prep HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Preparative)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
High‑Resolution Purification Platform
Ideal for final-stage purification workflows
Maximizes separation efficiency and product purity
Supports complex biomolecule separations
Method Development to Scale‑Up
Predictable selectivity when transitioning to longer columns or larger systems
Supports efficient analytical → semi‑prep → prep workflows
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs and batches
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load purification workflows
Reduced maintenance requirements
Typical Applications
Preparative biomolecule purification
Protein and peptide separations
High‑purity compound isolation
Pilot‑scale and production purification
Complex biomolecule mixtures
Pharmaceutical and QC workflows
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires preparative HPLC system capability (21.2 mm ID)
Ideal for maximum‑resolution purification workflows
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable