Prep HPLC Column, RP C18, Wide Pore, 5um, 300A. 21.2mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68518‑P21‑100‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is designed for biomolecule purification and preparative method development, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and consistent chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and reproducible purification performance
The 300 Å pore size enables large biomolecules to fully penetrate the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is widely used in analytical laboratories and pilot‑scale operations as a USP L1 alternate for large‑molecule separations.
100 mm Length – Increased Resolution for Biomolecule Development
Higher resolving power than 50 mm preparative scouting columns
Improved separation of proteins, peptides, and complex biomolecule mixtures
Increased peak capacity for purification workflows
Ideal for refining methods prior to longer columns or full scale‑up
This configuration is selected when greater resolution is required beyond initial preparative screening.
21.2 mm ID – High‑Capacity Preparative Scale
Provides very high sample loading capacity
Ideal for large‑scale biomolecule purification
Supports high‑flow preparative chromatography workflows
Typical Flow Rate Range
~10–30 mL/min (system dependent)
Optimize based on instrument capability and purification goals
✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes
Flow Rate & System Considerations (21.2 mm ID, 5 µm, 300 Å)
Requires systems capable of:
High flow rates
Large injection volumes
Compatible with:
Preparative pumps
High‑volume injection systems
Fraction collection systems
⚠️ Not intended for analytical or semi‑prep HPLC systems
Why Choose Wide Pore (300 Å) C18 (5 µm, Preparative)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
Method Development to Scale‑Up
Ideal for developing and refining biomolecule purification methods
Predictable selectivity when transitioning to longer columns or larger‑scale systems
Supports efficient process development workflows
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs and batches
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load purification workflows
Reduced maintenance requirements
Typical Applications
Preparative biomolecule purification
Protein and peptide separations
Pilot‑scale purification
Method development and optimization
Complex biomolecule mixtures
Pharmaceutical and QC workflows
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires preparative HPLC system capability (21.2 mm ID)
Ideal for method refinement prior to full‑scale purification
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable