Catalog Number - 68518-P21-100

The 68518‑P21‑100 Cogent RP C18 Preparative HPLC Column is designed for method development and preparative purification workflows, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, high loading capacity, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak shape for polar and basic analytes

• Consistent and reproducible purification results

This column is ideal as a USP L1 alternate and as a refinement platform for preparative method development and scale‑up workflows.

100 mm Length – Increased Resolution for Preparative Development

• Higher resolving power than 50 mm scouting columns

• Improved separation of closely related compounds

• Increased peak capacity for purification workflows

• Ideal for refining separations before scaling to longer columns

This configuration is selected when additional resolution is required beyond initial preparative scouting.

21.2 mm ID – High‑Capacity Preparative Scale

• Provides very high loading capacity for large‑scale purification

• Ideal for compound isolation and batch purification workflows

• Supports high‑flow preparative chromatography applications

Typical Flow Rate Range

• ~10–30 mL/min (system dependent)

• Optimize based on instrument capability and purification requirements

✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes

Flow Rate & System Considerations (21.2 mm ID, 5 µm)

• Requires systems capable of:

  • High flow rates

  • Large injection volumes

• Compatible with:

  • Preparative pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for analytical or semi‑prep HPLC systems

Why Choose RP C18 (5 µm, Preparative Scale)

Method Development to Scale‑Up

• Ideal for refining purification methods before final scale‑up

• Enables efficient method transfer across preparative formats

• Supports scalable process development workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids

• Provides greater retention than C8 phases

USP Method Compatibility

• Suitable for USP L1 methods

• Reliable for validated and regulated workflows

• Supports method transfer and consistency across systems

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for challenging analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between batches

Durable & Cost‑Efficient

• Long column lifetime

• Designed for high‑load purification cycles

• Reduced maintenance requirements

Typical Applications

• Preparative purification

• Method development and optimization

• Hydrophobic and neutral compound isolation

• Pharmaceutical and QC workflows

• Pilot‑scale purification

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for preparative HPLC systems (21.2 mm ID)

• Requires high‑flow capability and large sample loading capacity

• Ideal for method refinement prior to larger‑scale purification

• Optimize for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.