CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 5um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑25P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is designed for maximum separation performance of large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, improved recovery, and consistent chromatographic results.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Reliable and reproducible chromatography
The 300 Å pore size enables large molecules to fully interact with the stationary phase, minimizing restricted diffusion and reduced efficiency associated with standard 100 Å columns.
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the 4.0 mm wide‑pore C18 range
Maximizes peak capacity for highly complex biomolecule mixtures
Ideal for closely related proteins, peptides, and macromolecules
Provides extended retention for large and difficult analytes
Best suited for final method development and highest purity separations
This format is selected when shorter columns cannot achieve required resolution or separation goals.
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable chromatographic performance to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.85–1.10 mL/min
Benefits:
Lower solvent usage
Reduced operating cost
Compatible with standard HPLC systems
Flow Rate & System Considerations (4.0 mm ID, 5 µm, 300 Å)
Suitable for conventional HPLC systems
Supports a wide range of flow conditions
Well suited for:
Extended gradients
Complex analytical methods
Routine and high‑resolution workflows
Why Choose Wide Pore (300 Å) C18
Designed for Large Molecules
Enables efficient separation of:
Proteins
Peptides
Oligonucleotides
Eliminates mass transfer limitations of 100 Å stationary phases
Improved Recovery & Peak Shape
Greater analyte penetration into the stationary phase
Improved peak symmetry and reproducibility
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
RP C18 (5 µm) Performance Advantages
Robust & Reliable Operation
Ideal for routine analytical workflows and method development
Lower backpressure relative to smaller particle sizes
Suitable for long method lifetimes and consistent performance
Reliable Hydrophobic Retention
Strong retention for hydrophobic and neutral compounds
Effective for lipids, fatty acids, proteins, and peptides
Provides greater retention than C8 phases
Method Development & USP Compatibility
Ideal for new method development and optimization
Suitable for USP L1 wide‑pore methods for large molecules
Flexible across a wide range of analytes
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for polar and basic analytes
High Purity Silica
Consistent chromatographic results
Reduced variability between runs
Durable & Cost‑Efficient
Long column lifetime
Reduced system wear
Lower maintenance frequency
Typical Applications
Protein and peptide separations
Oligonucleotide analysis
Biomolecule HPLC and LC‑MS workflows
Lipids and hydrophobic compounds
Long‑chain fatty acids
Complex mixtures requiring maximum separation and purity
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Compatible with standard HPLC systems
Ideal for maximum‑resolution biomolecule analysis
Optimize method conditions to balance resolution and run time
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable