CUSTOM ITEM: HPLC Column, RP C18, 5um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑25P‑4 Cogent RP C18 HPLC Column is designed for maximum separation performance, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, improved peak symmetry, and consistent analytical results.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) chemistry, this stationary phase provides:
Complete end‑capping to minimize silanol interactions
Improved performance for polar and basic analytes
Consistent and reproducible chromatography across methods
This column is widely used as a starting point for new methods and as a USP L1 alternate for 5 µm analytical applications.
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the 4.0 mm RP C18 range
Maximizes peak capacity for highly complex mixtures
Ideal for closely related analytes and difficult separations
Provides longer retention for hydrophobic compounds
Best suited for final method optimization and highest purity separations
This format is selected when shorter columns do not provide sufficient resolution or retention.
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable chromatographic behavior to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.85–1.10 mL/min
Benefits:
Lower solvent usage
Reduced operating cost
Compatible with standard HPLC systems
Flow Rate & System Considerations (4.0 mm ID, 5 µm)
Suitable for conventional HPLC systems
Supports a wide range of flow conditions
Well suited for:
Viscous mobile phases
Long gradients
Extended analytical runs
Why Choose RP C18 (5 µm)
Robust & Reliable Operation
Ideal for routine analytical workflows and method development
Lower backpressure relative to smaller particle sizes
Suitable for long method lifetimes and consistent performance
Reliable Hydrophobic Retention
Strong reversed‑phase retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids
Provides greater retention than C8 phases
Method Development & USP Compatibility
Ideal for new method development and optimization
Suitable for USP L1 methods using 5 µm particles
Reliable alternate or replacement column
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for basic analytes
High Purity Silica
Consistent chromatographic performance
Reduced variability across runs
Durable & Cost‑Efficient
Long column lifetime
Reduced system wear
Lower maintenance frequency
Typical Applications
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Complex multi‑component mixtures
Method development and validation
USP L1 reversed‑phase workflows
Important Usage Notes
Compatible with standard HPLC systems
Ideal for maximum‑resolution analytical workflows
Best suited when separation performance and peak capacity are critical
Optimize method conditions to balance resolution and run time
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable