Catalog Number - 68518-25P-3M

The 68518‑25P‑3M Cogent RP C18 Wide Pore HPLC Column is engineered for maximum‑resolution separations of large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, improved recovery, and reproducible chromatographic performance.

Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and high‑MW analytes

• Consistent and reliable chromatography

The 300 Å pore structure allows macromolecules to fully interact with the stationary phase, minimizing restricted diffusion and reduced efficiency seen with standard 100 Å columns.

250 mm Length – Maximum Separation & Peak Capacity

• Highest resolving power in the 4.6 mm wide‑pore C18 range

• Maximizes peak capacity for complex biomolecule mixtures

• Ideal for proteins, peptides, and polysaccharides

• Provides extended retention for large and challenging analytes

• Best suited for final method development and highest purity separations

This format is selected when shorter columns cannot achieve the required level of separation or resolution.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column format

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• High reproducibility

• Easy method transfer across systems and laboratories

Flow Rate & System Considerations (4.6 mm ID, 5 µm, 300 Å)

• Suitable for standard HPLC systems

• Supports a wide range of flow and method conditions

• Well suited for:

  • Extended gradients

  • Complex analytical methods

  • Routine and high‑resolution workflows

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables efficient separation of:

  • Proteins

  • Peptides

  • Polysaccharides

• Eliminates mass transfer limitations of 100 Å stationary phases

Improved Recovery & Peak Shape

• Better analyte penetration into the stationary phase

• Improved peak symmetry and reproducibility

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (5 µm) Performance Advantages

Robust & Reliable Operation

• Ideal for routine analytical workflows and method development

• Lower backpressure relative to smaller particle sizes

• Suitable for long method lifetimes and consistent performance

Reliable Hydrophobic Retention

• Strong retention for hydrophobic and neutral compounds

• Effective for lipids, fatty acids, proteins, and peptides

• Provides greater retention than C8 phases

Method Development Versatility

• Ideal for new method development and optimization

• Suitable for USP L1 wide‑pore applications

• Flexible across diverse analyte types

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Reduced system wear

• Lower maintenance frequency

Typical Applications

• Protein and peptide separations

• Polysaccharide analysis

• Biomolecule HPLC workflows

• UV or ELSD detection methods

• Lipids and hydrophobic compounds

• Complex mixtures requiring maximum separation and purity

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Compatible with standard HPLC systems

• Often used with UV or ELSD detection for macromolecules

• Ideal for maximum‑resolution biomolecule analysis