HPLC Column, RP C18, 5um, 2.1mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑25P‑2 Cogent RP C18 HPLC Column is engineered for maximum separation performance in LC‑MS and analytical workflows, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, excellent peak shape, and consistent results.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:
Complete end‑capping to minimize silanol interactions
Improved peak symmetry for polar and basic analytes
Consistent, reproducible chromatography across demanding methods
This column is a trusted choice for USP L1 method substitution, method development, and high‑resolution LC‑MS workflows.
250 mm Length – Maximum Resolution & Peak Capacity
Highest resolving power in the 2.1 mm RP C18 range
Maximizes peak capacity for complex mixtures
Ideal for closely related compounds and trace-level separations
Provides extended retention for hydrophobic analytes
Best suited for final method optimization and high‑purity analysis
This configuration is selected when shorter columns cannot provide sufficient resolution or separation.
Flow Rate & System Considerations (2.1 mm ID, 5 µm)
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.20–0.30 mL/min
Requires:
HPLC or LC‑MS system capable of stable low‑flow delivery
Low‑volume detector flow cell for optimal sensitivity
Precision injection system
⚠️ Systems must maintain accurate and consistent low‑flow performance for best results
Why Choose RP C18 (5 µm
Robust & Reliable Operation
Ideal for routine analytical workflows and method development
Lower backpressure relative to smaller particle sizes
Suitable for long, reproducible analytical methods
Reliable Hydrophobic Retention
Strong reversed‑phase retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids
Provides greater retention than C8 phases
Method Development & USP Compatibility
Ideal for new method development and optimization
Suitable for USP L1 methods using 5 µm particles
Reliable alternate or replacement column
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances performance for polar and basic analytes
High Carbon Load
Increased retention and loading capacity
Greater flexibility across a wide range of analytes
Low Column Bleed
Suitable for sensitive detection methods (LC‑MS and UV)
Cleaner baselines and improved data quality
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs and batches
Typical Applications
LC‑MS analytical workflows
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Complex multi‑component mixtures
Method development and validation
USP L1 reversed‑phase methods
Important Usage Notes
Designed for low‑flow analytical workflows (2.1 mm ID)
Requires stable low‑flow system capability
Ideal for maximum‑resolution separations and complex mixtures
Optimize flow rate and gradient conditions for best performance