CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 5um, 4.0mm ID x 150mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑15P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is engineered for maximum‑resolution separations of large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte access, improved recovery, and consistent chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Excellent reproducibility and durability
The 300 Å pore size enables large molecules to fully interact with the stationary phase, eliminating restricted diffusion and reduced recovery seen with standard 100 Å columns.
150 mm Length – Maximum Resolution & Peak Capacity
Highest resolving power in the 4.0 mm wide‑pore C18 range
Maximizes peak capacity for complex biomolecule mixtures
Ideal for closely related proteins, peptides, and macromolecules
Preferred for final method optimization and high‑purity separations
Longer run times compared to 50 mm and 100 mm formats
This configuration is best suited when maximum separation performance is required over speed.
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable chromatographic behavior to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.85–1.10 mL/min
Benefits:
Lower solvent usage
Reduced operating cost
Compatible with standard HPLC systems
Flow Rate & System Considerations (4.0 mm ID, 5 µm, 300 Å)
Suitable for conventional HPLC systems
Supports higher flow rates compared to smaller particle sizes
Appropriate for:
Viscous mobile phases
Extended gradients
Routine and demanding analytical workflows
Why Choose Wide Pore (300 Å) C18
Designed for Large Molecules
Enables efficient separation of:
Proteins
Peptides
Oligonucleotides
Eliminates mass transfer limitations of 100 Å stationary phases
Improved Recovery & Peak Shape
Greater analyte penetration into stationary phase
Improved peak symmetry and reproducibility
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
RP C18 (5 µm) Performance Advantages
Robust & Reliable Operation
Ideal for routine analytical workflows and method development
Lower backpressure relative to smaller particle sizes
Well suited for long method lifetimes and consistent performance
Reliable Hydrophobic Retention
Strong retention for hydrophobic and neutral compounds
Effective for lipids, fatty acids, proteins, and peptides
Provides greater retention than C8 phases
Method Development Versatility
Ideal for new method development and optimization
Suitable for USP L1 wide‑pore applications
Flexible across a wide range of analytes
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for polar and basic analytes
High Purity Silica
Consistent chromatographic results
Reduced variability between runs
Durable & Cost‑Efficient
Long column lifetime
Reduced system strain
Lower maintenance frequency
Typical Applications
Protein and peptide separations
Oligonucleotide analysis
Biomolecule HPLC and LC‑MS workflows
Lipids and hydrophobic compounds
Long‑chain fatty acids
Complex biomolecule mixtures requiring maximum resolution
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Compatible with standard HPLC systems
Ideal for applications requiring maximum resolution and recovery
Optimize flow rate and mobile phase to balance resolution and run time
Custom Order Information
This product is a made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable
Please contact Customer Service for lead time and ordering assistance.