Catalog Number - 68518-15P-3M

The 68518‑15P‑3M Cogent RP C18 Wide Pore HPLC Column is designed for maximum‑resolution separations of large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure to deliver improved analyte access, peak shape, and reproducibility.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible chromatographic performance

The 300 Å pore size allows macromolecules to fully access the stationary phase, eliminating restricted diffusion and poor recovery seen with standard 100 Å columns.

150 mm Length – Maximum Resolution & Peak Capacity

• Highest resolving power in the 4.6 mm wide‑pore C18 range

• Maximizes peak capacity for complex biomolecule mixtures

• Ideal for proteins, peptides, and polysaccharides

• Preferred for analytical workflows requiring high purity separations

• Longer run times compared to 50 mm and 100 mm formats

This configuration is used when maximum separation performance outweighs speed.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column dimension

• Compatible with conventional analytical systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• Reliable reproducibility

• Easy method transfer across systems

Flow Rate & System Considerations (4.6 mm ID, 5 µm, 300 Å)

• Suitable for standard HPLC systems

• Supports higher flow rates compared to smaller particle sizes

• Well suited for:

  • Viscous mobile phases

  • Extended gradients

  • Routine and complex analytical workflows

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables efficient separation of:

  • Proteins

  • Peptides

  • Polysaccharides

• Eliminates mass transfer limitations of 100 Å stationary phases

Improved Recovery & Peak Shape

• Better analyte penetration into the stationary phase

• Improved peak symmetry and reproducibility

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (5 µm) Performance Advantages

Robust & Reliable Operation

• Ideal for routine analytical workflows and method development

• Lower backpressure relative to smaller particle sizes

• Suitable for long method lifetimes and reproducible results

Reliable Hydrophobic Retention

• Strong retention for hydrophobic and neutral compounds

• Effective for lipids, fatty acids, proteins, and peptides

• Provides greater retention than C8 phases

Method Development Versatility

• Ideal for new method development and optimization

• Suitable for USP L1 wide‑pore applications

• Flexible across diverse analyte classes

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatographic results

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Reduced system strain

• Lower maintenance frequency

Typical Applications

• Protein and peptide separations

• Polysaccharide analysis

• Biomolecule HPLC workflows

• LC‑MS with flow splitting

• Lipids and hydrophobic compounds

• Complex mixtures requiring maximum resolution

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Compatible with standard HPLC systems

• Often used with UV detection or LC‑MS with flow splitting

• Ideal for applications requiring high‑resolution biomolecule separations