Catalog Number - 68518-15P-2-3M

The 68518‑15P‑2‑3M Cogent RP C18 Wide Pore HPLC Column is designed for maximum‑resolution separations of large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure for enhanced analyte accessibility and chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible chromatographic performance

The 300 Å pore size allows large molecules to fully access the stationary phase, eliminating restricted diffusion and poor recovery associated with standard 100 Å columns.

150 mm Length – Maximum Resolution for Biomolecules

• Highest resolving power in the 2.1 mm wide‑pore C18 range

• Maximizes peak capacity for complex biomolecule mixtures

• Ideal for closely related proteins, peptides, and macromolecules

• Preferred for LC‑MS workflows requiring detailed separation

• Longer run times compared to 50 mm and 100 mm formats

This format is selected when maximum separation and purity are required over speed.

Flow Rate & System Considerations (2.1 mm ID, 5 µm, 300 Å)

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.20–0.30 mL/min

Requires:

• HPLC or LC‑MS system capable of stable low‑flow delivery

• Low‑volume detector flow cell

• Precision injection system

⚠️ Systems must be capable of reliable low‑flow operation for 2.1 mm ID columns

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables efficient separation of:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates mass transfer limitations of 100 Å stationary phases

Improved Recovery & Peak Shape

• Better analyte penetration into the stationary phase

• Improved peak symmetry and reproducibility

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (5 µm) Performance Advantages

Robust & Reliable Operation

• Ideal for routine analytical workflows and method development

• Lower backpressure relative to smaller particle sizes

• Suitable for a wide range of HPLC systems and methods

Reliable Hydrophobic Retention

• Strong retention for hydrophobic and neutral compounds

• Effective for lipids, fatty acids, proteins, and peptides

• Provides greater retention than C8 phases

Method Development Versatility

• Ideal for LC‑MS method development and optimization

• Suitable for USP L1 wide‑pore applications

• Flexible across a wide range of analytes

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatographic results

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Reduced system strain

• Lower maintenance frequency

Typical Applications

• LC‑MS biomolecule workflows

• Protein and peptide separations

• Oligonucleotide analysis

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

• Complex biomolecule mixtures requiring maximum resolution

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Requires stable low‑flow system performance (2.1 mm ID)

• Ideal when maximum resolution is required for biomolecular analysis

• Optimize temperature and mobile phase for best performance