HPLC Column, RP C18, 5um, 2.1mm ID x 150mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑15P‑2 Cogent RP C18 HPLC Column is a durable, reversed‑phase column designed for maximum‑resolution analytical separations, featuring 5 µm particles and fully double end‑capped C18 bonding for consistent retention and reliable performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) chemistry, this stationary phase:
Uses complete end‑capping to minimize silanol interactions
Improves peak shape for polar and basic analytes
Provides robust and reproducible chromatography
This column is ideal as a USP L1 alternate column and a dependable solution for routine analysis and high‑resolution LC‑MS workflows.
150 mm Length – Maximum Resolution for LC‑MS
Highest resolving power in the 2.1 mm RP C18 (5 µm) range
Maximizes peak capacity for complex mixtures
Ideal for closely related analytes and difficult separations
Preferred for LC‑MS workflows requiring detailed resolution
Longer run times compared to 50 mm and 100 mm formats
This format is best suited when maximum separation and purity are prioritized over speed.
Flow Rate & System Requirements (2.1 mm ID, 5 µm)
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.20–0.30 mL/min for maximum resolution
Requires:
HPLC or LC‑MS system capable of stable low‑flow delivery
Low‑volume detector flow cell
Precision injection system
✅ Operates under standard high‑pressure HPLC conditions ✅ Produces lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure analytical column
Why Choose RP C18 (5 µm)
High‑Pressure Robustness & Stability
Designed for standard HPLC high‑pressure operation
Lower backpressure relative to smaller particle sizes
Ideal for rugged, long‑term analytical methods
Reliable Hydrophobic Retention
Strong reversed‑phase retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids
Provides longer retention than C8 phases
Method Development & USP Compatibility
Ideal for method development and optimization
Suitable for USP L1 methods using 5 µm particle columns
Reliable alternate or replacement column
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances performance for basic analytes
High Purity Silica
Consistent chromatographic results
Reduced variability across runs and columns
Durable & Cost‑Efficient
Longer column lifetime vs smaller particle sizes
Reduced system strain
Lower maintenance and replacement costs
Typical Applications
LC‑MS analytical workflows
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Complex mixture separations
USP L1 reversed‑phase methods
Important Usage Notes
Operates under standard high‑pressure HPLC conditions
Requires stable low‑flow capability for 2.1 mm ID columns
Ideal for maximum‑resolution analytical workflows
Best suited when complex separations require greater peak capacity